High levels of a major histocompatibility complex II-self peptide complex on dendritic cells from the T cell areas of lymph nodes.
High levels of a major histocompatibility complex II-self peptide complex on dendritic cells from the T cell areas of lymph nodes.
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来自淋巴结T细胞区域的树突状细胞上的主要组织相容性复合物II并肽复合物。
DOI：
10.1084/jem.186.5.665
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发表时间：
1997-08-29
期刊：
影响因子：
--
通讯作者：
Steinman RM
中科院分区：
文献类型：
--
作者：
Inaba K;Pack M;Inaba M;Sakuta H;Isdell F;Steinman RM
T 淋巴细胞通过外周淋巴结的 T 细胞区域不断再循环。在每次传代过程中，T 细胞都会检查大树突细胞 (DC)（也称为叉指细胞）的表面。然而，这些 DC 很难从淋巴结中释放出来。通过强调使用无钙培养基，如 Vremec 等人所示。 （Vremec, D., M. Zorbas, R. Scollay, D.J. Saunders, C.F. Ardavin, L. Wu, and K. Shortman. 1992. J. Exp. Med. 176:47–58.），我们已经能够从 T 细胞区域释放并富集 DC。 DC 表达 CD11c 白细胞整联蛋白、用于抗原呈递的 DEC-205 多凝集素受体、单克隆抗体 M342、2A1 和 MIDC-8 识别的细胞内颗粒抗原、非常高水平的 MHC I 和 MHC II 以及丰富的辅助分子，例如 CD40、CD54 和 CD86。当用识别 I-Ab 和 I-Eα 衍生肽之间形成的复合物的 Y-Ae 单克隆抗体进行检查时，T 细胞区域 DC 表达最高水平。富集的 DC 还刺激了对该 MHC II-肽复合物具有特异性的 T-T 杂交瘤，并且该杂交瘤经历了细胞凋亡。因此，可以分离 T 细胞区域内的 DC。因为它们呈现非常高水平的自身肽，所以在外周自身反应性的调节中应考虑这些 DC。
T lymphocytes recirculate continually through the T cell areas of peripheral lymph nodes. During each passage, the T cells survey the surface of large dendritic cells (DCs), also known as interdigitating cells. However, these DCs have been difficult to release from the lymph node. By emphasizing the use of calcium-free media, as shown by Vremec et al. (Vremec, D., M. Zorbas, R. Scollay, D.J. Saunders, C.F. Ardavin, L. Wu, and K. Shortman. 1992. J. Exp. Med. 176:47–58.), we have been able to release and enrich DCs from the T cell areas. The DCs express the CD11c leukocyte integrin, the DEC-205 multilectin receptor for antigen presentation, the intracellular granule antigens which are recognized by monoclonal antibodies M342, 2A1, and MIDC-8, very high levels of MHC I and MHC II, and abundant accessory molecules such as CD40, CD54, and CD86. When examined with the Y-Ae monoclonal which recognizes complexes formed between I-Ab and a peptide derived from I-Eα, the T cell area DCs expressed the highest levels. The enriched DCs also stimulated a T-T hybridoma specific for this MHC II–peptide complex, and the hybridoma underwent apoptosis. Therefore DCs within the T cell areas can be isolated. Because they present very high levels of self peptides, these DCs should be considered in the regulation of self reactivity in the periphery.