The effects of inhibitors of RNA and protein synthesis on chloroplast structure and function in wild-type Chlamydomonas reinhardi.
The effects of inhibitors of RNA and protein synthesis on chloroplast structure and function in wild-type Chlamydomonas reinhardi.
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RNA和蛋白质合成抑制剂对野生型衣原体中叶绿体结构和功能的影响。
DOI：
10.1083/jcb.50.1.35
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发表时间：
1971-07
影响因子：
7.8
通讯作者：
Goodenough, U W
中科院分区：
文献类型：
--
作者：
Goodenough, U W
单细胞绿色藻类莱茵衣藻的野生型细胞已在利福平（一种叶绿体DNA依赖性RNA聚合酶的抑制剂）、大观霉素和氯霉素（两种叶绿体核糖体蛋白质合成抑制剂）和环己酰亚胺（一种细胞质核糖体蛋白质合成抑制剂）的存在下生长了几代。放线菌酮的影响是复杂的，它的结论是，这种抑制剂不能提供有意义的信息细胞质控制的叶绿体成分的合成在长期的实验与C。reinhardi。在乙酸盐存在下，并在适当的浓度下，叶绿体蛋白质合成的三种抑制剂只稍微延缓生长速率，不影响叶绿素的合成;然而，光合速率降低四倍后，几代的增长。每种抑制剂在叶绿体膜的组织中产生相似的损伤模式。只有利福平阻止叶绿体核糖体的产生。
Wild-type cells of the unicellular green alga Chlamydomonas reinhardi have been grown for several generations in the presence of rifampicin, an inhibitor of chloroplast DNA-dependent RNA polymerase, spectinomycin and chloramphenicol, two inhibitors of protein synthesis on chloroplast ribosomes, and cycloheximide, an inhibitor of protein synthesis on cytoplasmic ribosomes. The effects of cycloheximide are complex, and it is concluded that this inhibitor cannot give meaningful information about the cytoplasmic control over the synthesis of chloroplast components in long-term experiments with C. reinhardi. In the presence of acetate and at the appropriate concentrations, the three inhibitors of chloroplast protein synthesis retard growth rates only slightly and do not affect the synthesis of chlorophyll; however, photosynthetic rates are reduced fourfold after several generations of growth. Each inhibitor produces a similar pattern of lesions in the organization of chloroplast membranes. Only rifampicin prevents the production of chloroplast ribosomes.