A fluorescent probe for fast and quantitative detection of hydrogen sulfide in blood.
A fluorescent probe for fast and quantitative detection of hydrogen sulfide in blood.
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DOI：
10.1002/anie.201104236
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发表时间：
2011-10-04
影响因子：
16.6
通讯作者：
Wang, Binghe
中科院分区：
文献类型：
--
作者：
Peng, Hanjing;Cheng, Yunfeng;Dai, Chaofeng;King, Adrienne L.;Predmore, Benjamin L.;Lefer, David J.;Wang, Binghe
硫化氢（H2S）以其难闻的臭鸡蛋味而闻名，传统上被认为是一种有毒气体。然而，最近的研究表明，硫化氢是一种内源性产生的气体信号化合物（气体递质），其重要性与其他两种已知的内源性气体递质，一氧化氮（NO）[1]和一氧化碳（CO）[2]相当H2S已被公认为介导广泛的生理效应。研究表明H2S可以作为K-ATP通道开启剂对心血管系统产生影响一些研究表明H2S在心肌缺血等情况下的保护作用，很可能是通过其抗氧化和抗凋亡信号作用的结合进一步的研究也表明H2S可能对缺血性心力衰竭的治疗有疗效。[6,7]也是中枢神经系统、[8-10]呼吸系统、胃肠系统和内分泌系统的调制剂硫化氢似乎表现出NO几乎所有的有益作用，而不产生有毒的活性氧（ROS）。此外，它还可以作为ROS的抗氧化剂或清除剂。此外，研究表明，硫化氢水平与唐氏综合症[12]和阿尔茨海默病[13]等疾病有关因此，近年来人们对硫化氢的生理和病理功能的研究越来越感兴趣。[11,14,15]研究硫化氢的一个重要限制因素是缺乏能够快速准确检测硫化氢的传感器和试剂。比色法、[16-18]电化学分析、[19-21]和气相色谱法等方法均有报道。[22,23]然而，众所周知，硫化氢的分解代谢非常快，这可能导致其浓度持续波动，导致难以准确分析这一重要分子。目前的方法不允许快速、准确和实时的测定。不同的内源性硫化物浓度已被报道，大多数出版物表明血液中的硫化物浓度在10-100毫米范围内。[24-29]也有其他研究表明硫化物浓度要低得多。[30,31]因此，需要新的方法来有效检测生物系统中的硫化物。为了开发一种在生理条件下快速测定硫化氢浓度的新方法，我们致力于为这一重要的气体递质寻找一种选择性化学感测剂。为了便于在生物学实验室中使用，化学感测剂应1)在温和条件下快速（几秒钟内）起作用，2)化学稳定，可长期储存，3)在接近生理条件下检测灵敏，4)在生理相关的硫化氢浓度范围内显示线性浓度-信号关系，以便于定量，5)血液中其他阴离子的干扰最小或不干扰。6)在水溶液和血浆中起作用。本文报道了一种荧光化学探针的研制及其在测定水溶液、血清和全血中硫化氢中的应用。荧光是最灵敏的检测方法之一。因此，我们有兴趣选择一种具有高量子产率，在长波长发射，并通过荧光性质变化响应硫化氢的荧光团。丹酚是一种常用的荧光团，以其强荧光和长发射而闻名。
Hydrogen sulfide (H2S), well-known for its unpleasant rotten egg smell, was traditionally considered as a toxic gas. However, recent studies have demonstrated that hydrogen sulfide is an endogenously produced gaseous signaling compound (gasotransmitter) with importance on a par with that of the other two known endogenous gasotransmitters, nitric oxide (NO)[1] and carbon monoxide (CO).[2] H2S has been recognized for mediating a wide range of physiological effects. Studies have shown that H2S can have an effect on the cardiovascular system [3] by acting as a K-ATP channel opener.[4] Several studies have shown the protective roles of H2S, in situations such as myocardial ischemia, most likely through a combination of its antioxidant and anti-apoptotic signaling effects.[5] Further studies also showed that H2S may be of therapeutic benefit for the treatment of ischemiainduced heart failure.[6, 7] It is also a modulator in the central nervous system,[8–10] respiratory system, gastrointestinal system, and endocrine system.[11] It seems that hydrogen sulfide exhibits almost all the beneficial effects of NO without generating the toxic reactive oxygen species (ROS). In addition, it also acts as an antioxidant or scavenger of ROS. Furthermore, research has indicated that the hydrogen sulfide level is related to diseases such as the Down syndrome [12] and Alzheimer s disease.[13] Therefore, recent years have seen a steady increase in interest in understanding the physiological and pathological functions of hydrogen sulfide.[11, 14, 15] One significant limiting factor in studying hydrogen sulfide is the lack of sensors and agents that allow for its rapid and accurate detection. Methods using colorimetric,[16–18] electrochemical analysis,[19–21] and gas chromatography have been reported.[22, 23] However, catabolism of hydrogen sulfide is known to be fast, which could result in continuous fluctuation in its concentration, leading to difficulties in the accurate analysis of this important molecule. Current methods do not allow for fast, accurate, and real-time determination. Different endogenous sulfide concentrations have been reported with most publications suggesting the sulfide concentration in blood is in the range of 10–100 mm.[24–29] There have been other studies suggesting much lower sulfide concentrations.[30, 31] Therefore, new methods are needed for the efficient detection of sulfide in biological systems. With the idea of developing a new method that will be useful for rapid assay of hydrogen sulfide concentrations under physiological conditions, we undertook the effort of searching for a selective chemosensing agent for this important gasotransmitter. For easy use in a biology laboratory, the chemosensing agent should 1) act fast (within seconds) under mild conditions, 2) be chemically stable for long-term storage, 3) be sensitive for detection under near physiological conditions, 4) show a linear concentration–signal relationship within physiologically relevant hydrogen sulfide concentration ranges for easy quantitation, 5) show minimal or no interference by other anions in the blood, and 6) be functional in aqueous solutions and blood plasma. Herein, we report the development of a fluorescent chemoprobe and its application in the determination of hydrogen sulfide in aqueous solution, serum, and whole blood.Fluorescence is one of the most sensitive detection methods. Thus we were interested in selecting a fluorophore, which has a high quantum yield, emits at a long wavelength, and responds to hydrosulfide by fluorescent property changes. Dansyl is a commonly used fluorophore, and well-known for its strong fluorescence and long emission …
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影响因子：
20.1
作者：
Calvert JW;Jha S;Gundewar S;Elrod JW;Ramachandran A;Pattillo CB;Kevil CG;Lefer DJ
通讯作者：
Lefer DJ
影响因子：
37.8
作者：
Calvert JW;Elston M;Nicholson CK;Gundewar S;Jha S;Elrod JW;Ramachandran A;Lefer DJ
通讯作者：
Lefer DJ
影响因子：
9.6
作者：
Chen, YH;Yao, WZ;Tang, CS
通讯作者：
Tang, CS
影响因子：
56.9
作者：
CULOTTA, E;KOSHLAND, DE
通讯作者：
KOSHLAND, DE
DOI：
10.1073/pnas.0705891104
发表时间：
2007-09-25
影响因子：
11.1
作者：
Elrod, John W.;Calvert, John W.;Lefer, David J.
通讯作者：
Lefer, David J.