A CIS-ACTING TRANSCRIPTION ELEMENT OF THE C-MYC GENE CAN ASSUME AN H-DNA CONFORMATION
A CIS-ACTING TRANSCRIPTION ELEMENT OF THE C-MYC GENE CAN ASSUME AN H-DNA CONFORMATION
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DOI：
10.1093/nar/17.19.7771
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发表时间：
1989-10-11
影响因子：
14.9
通讯作者：
KINNIBURGH, AJ
中科院分区：
文献类型：
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作者：
KINNIBURGH, AJ
我已经使用化学探针和一个c-myc的DNA区域的核酸酶敏感，c-myc的DNA区域的结构确定的一个c-myc的关联分析。我发现这个DNA区域在体外可以形成三链-单链构象，即H-DNA构象。该DNA区域先前已被证明是c-myc基因的正性顺式作用转录元件，并结合核因子，包括碱基配对的核糖核蛋白10。因此，H-DNA可能是一个重要的功能在体内拓扑异构体的H-DNA和B-DNA构象具有不同的转录活性。
I have used chemical probes and an oligonucleotide-association assay to determine the structure of a nuclease-sensitive, c-myc DNA region. I find that this DNA region can form a triplex-single stranded conformer in vitro.sbd.the H-DNA conformer. This DNA region has been shown previously to be a positive, cis-acting transcription element of the c-myc gene and to bind nuclear factors, incluing a base-paired ribonucleoprotein10. Therefore, H-DNA may be a functionally important in vivo toposiomer where the H-DNA and B-DNA conformers have different transcriptional activities.