Description of an in situ hybridization methodology for detection of Epstein-Barr virus RNA in paraffin-embedded tissues, with a survey of normal and neoplastic tissues.
Description of an in situ hybridization methodology for detection of Epstein-Barr virus RNA in paraffin-embedded tissues, with a survey of normal and neoplastic tissues.
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DOI：
10.1097/00019606-199212000-00005
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发表时间：
1992-12-01
期刊：
影响因子：
--
通讯作者：
Weiss, L M
中科院分区：
文献类型：
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作者：
Chang, K L;Chen, Y Y;Weiss, L M
作者描述了一种高灵敏度和实用的原位杂交方法，使用EBER1 RNA的寡核苷酸探针检测福尔马林固定，石蜡包埋的组织切片中的EB病毒（EBV）。本文对793例正常组织和肿瘤组织石蜡包埋标本进行了研究。在多种已知的EBV阳性肿瘤中，EBV RNA的核染色均匀地存在于所有或几乎所有的肿瘤细胞中。我们还证明了正常淋巴组织中罕见的EBV感染细胞。RNA酶预消化、竞争性抑制和对照探针研究证实了染色的特异性。此外，EBV RNA染色与其他病毒的交叉反应性不存在。此外，EBV在其他各种正常和肿瘤组织中的分布也有报道。
The authors describe a highly sensitive and practical in situ hybridization method using an oligonucleotide probe for EBER1 RNA for the detection of Epstein-Barr virus (EBV) in formalin-fixed, paraffin-embedded tissue sections. Paraffin-embedded tissues from 793 cases of normal and neoplastic tissues were studied. Nuclear staining for EBV RNA was uniformly present in all or virtually all neoplastic cells in a variety of known EBV-positive tumors. We also demonstrate rare EBV-infected cells in normal lymphoid tissues. RNAase predigestion, competitive inhibition, and control probe studies confirmed the specificity of the staining. In addition, cross-reactivity of EBV RNA staining with other viruses was not present. Additionally, the distribution of EBV in a wide variety of other normal and neoplastic tissues is reported.