HYPOTHALAMIC NEURONAL HISTAMINE REGULATES FEEDING CIRCADIAN-RHYTHM IN RATS
HYPOTHALAMIC NEURONAL HISTAMINE REGULATES FEEDING CIRCADIAN-RHYTHM IN RATS
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DOI：
10.1016/0006-8993(94)90160-0
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发表时间：
1994-04-04
期刊：
影响因子：
2.9
通讯作者：
NIKI, N
中科院分区：
文献类型：
--
作者：
DOI, T;SAKATA, T;NIKI, N
为了阐明下丘脑神经元组胺在喂养昼夜节律中的作用，我们使用影响内源性组胺活性的化学探针分析了大鼠的行为模式。α -氟甲基组氨酸（FMH）是一种组胺合成酶的特异性自杀抑制剂，持续输注到大鼠的第三脑室会破坏进食、饮水和走动行为的光-暗周期。光照期12 h的摄食量和饮水量增加，暗期12 h的摄食量和活动减少。光照周期与24 h总周期之比（L/T ratio）在各行为参数中均有所增加。通过3-h累积分析评估，FMH输注后，所有行为参数的昼夜节律幅度均下降，而只有动态活动的前端期前移。氯苯那敏，一种h -1拮抗剂。有选择地在光照期增加食物摄入量，在黑暗期减少食物摄入量。因此，拮抗剂增加了食物摄取量的L/T比，但对水摄取量和运动活性的比例没有影响。法莫替丁，一种h -2拮抗剂，在任何参数中都不影响比值。硫哌丁胺是一种对突触前h -3受体位点的组胺合成和释放具有自抑制作用的拮抗剂，在黑暗中减少了食物摄入量，但对L/T比的任何参数都没有影响。这些发现提示神经元组胺可能通过下丘脑组胺h -1受体调节大鼠的摄食昼夜节律。
To clarify involvement of hypothalamic neuronal histamine in feeding circadian rhythm, we analyzed rat behavioral patterns using chemical probes which affect endogenous histaminergic activity. Sustained infusion of alpha-fluoromethylhistidine (FMH), a specific suicide inhibitor of a histamine-synthesizing enzyme, into the rat third cerebral ventricle disrupted light-dark cycles of feeding, drinking, and ambulatory behavior. Food and water intake and ambulatory activity during the 12-h light period increased, and those during the 12-h dark period decreased after the infusion. The ratio of the light period to the 24-h total period (L/T ratio) increased in all behavioral parameters. Assessed by 3-h cumulative analysis, amplitudes of circadian rhythmicity decreased in all behavioral parameters, whereas only the acrophase of ambulatory activity shifted forward after FMH infusion. Chlorpheniramine, an H-1-antagonist. selectively increased food intake during the light and decreased it during the dark period. Consequently, the antagonist increased the L/T ratio in food intake, but did not affect the ratio in water intake or ambulatory activity. Famotidine, an H-2-antagonist, did not affect the ratio in any parameter. Thioperamide, an antagonist of auto-inhibitory effects on histamine synthesis and release at presynaptic H-3-receptor sites, decreased food intake during the dark, but did not affect the L/T ratio in any parameter. These findings indicate that neuronal histamine may regulate feeding circadian rhythm through the hypothalamic histamine H-1-receptor in rats.