Uptake of proteinase-α-macroglobulin complexes by macrophages
Uptake of proteinase-α-macroglobulin complexes by macrophages
复制标题
巨噬细胞摄取蛋白酶-α-巨球蛋白复合物
DOI：
10.1016/0304-4165(75)90309-8
复制
发表时间：
1975
期刊：
影响因子：
--
通讯作者：
J. Dolovich
中科院分区：
文献类型：
--
作者：
M. Debanne;R. Bell;J. Dolovich
标记的蛋白酶（亚替洛肽酶A、胰蛋白酶）与血清α1-巨球蛋白或α2-巨球蛋白的复合物在体外可被兔肺泡巨噬细胞和腹腔巨噬细胞迅速吸收，但不能被混合的兔外周血白细胞吸收。不与α1-或α2巨噬蛋白结合的酶不与肺泡巨噬细胞相关。化学灭活的亚替洛肽酶A不与α1-或α2微球蛋白结合，不与肺泡巨噬细胞相互作用。阻断实验证实，蛋白酶与肺泡巨噬细胞的相互作用具有复杂的特异性；同时加入由未标记的亚替洛肽酶A、亚替洛肽酶B、胰蛋白酶或凝乳胰蛋白酶形成的巨球蛋白复合物可阻止标记复合物的摄取，但单独加入巨球蛋白不能阻止。研究结果表明，蛋白酶-α-巨球蛋白复合物与巨噬细胞之间存在复合物特异性相互作用。
Complexes of labelled proteinases (subtilopeptidase A, trypsin) with serum α1-macroglobulin or α2-macroglobulin are rapidly taken up in vitro by rabbit alveolar macrophages and peritoneal macrophages but not by mixed rabbit peripheral blood leukocytes. Enzyme, not bound to α1- or α2-macrogobulin, does not become associated with alveolar macrophages. Chemically inactivated subtilopeptidase A does not bind to α1- or α2-microglobulin, does not interact with alveolar macrophages. Blocking experiments confirmed that the interaction of proteinase with alveolar macrophages is complex specific; uptake of labelled complex was prevented by the simultaneous addition of macroglobulin complexes formed with non-labelled subtilopeptidase A, subtilopeptidase B, trypsin or chymotrypsin but not by macroglobulin alone. The findings demonstrate a complex-specific interaction between proteinase-α-macroglobulin complexes and macrophages.