One step fabrication of hydrogel microcapsules with hollow core for assembly and cultivation of hepatocyte spheroids.
One step fabrication of hydrogel microcapsules with hollow core for assembly and cultivation of hepatocyte spheroids.
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DOI：
10.1016/j.actbio.2017.01.010
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发表时间：
2017-03-01
影响因子：
9.7
通讯作者：
Revzin A
中科院分区：
文献类型：
--
作者：
Siltanen C;Diakatou M;Lowen J;Haque A;Rahimian A;Stybayeva G;Revzin A
3D肝脏微组织可以作为有价值的肝脏类似物，用于基于细胞的治疗和临床前药物开发期间的肝毒性筛选。然而，肝细胞在体外快速去分化，并且通常需要3D培养系统或共培养物用于表型拯救。在这项工作中，我们提出了一种新的微胶囊化策略，利用同轴流动聚焦液滴微流体技术，以制造微胶囊与液体核心和聚（乙二醇）（PEG）的外壳。原代肝细胞被包裹在这些囊内后，迅速形成细胞-细胞接触，并组装成致密的球状体。高水平的肝功能在胶囊内维持了十多天。这里描述的微囊化方法与难以培养的原代上皮细胞相容，允许调整凝胶机械性能和扩散性，并且可以在未来用于高密度悬浮细胞培养。
3D hepatic microtissues can serve as valuable liver analogues for cell-based therapies and for hepatotoxicity screening during preclinical drug development. However, hepatocytes rapidly dedifferentiate in vitro, and typically require 3D cultures systems or co-cultures for phenotype rescue. In this work we present a novel microencapsulation strategy, utilizing coaxial flow-focusing droplet microfluidics to fabricate microcapsules with liquid core and poly(ethylene glycol) (PEG) shell. When entrapped inside these capsules, primary hepatocytes rapidly formed cell-cell contacts and assembled into compact spheroids. High levels of hepatic function were maintained inside the capsules for over ten days. The microencapsulation approach described here is compatible with difficult-to-culture primary epithelial cells, allows for tuning gel mechanical properties and diffusivity, and may be used in the future for high density suspension cell cultures.
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影响因子：
4
作者：
Huang, Haishui;He, Xiaoming
通讯作者：
He, Xiaoming
影响因子：
4.1
作者：
Li, Cheri Y.;Stevens, Kelly R.;Bhatia, Sangeeta N.
通讯作者：
Bhatia, Sangeeta N.
影响因子：
6
作者：
Foster, Elena;You, Jungmok;Revzin, Alexander
通讯作者：
Revzin, Alexander
影响因子：
14
作者：
Hwang, Yu-Shik;Cho, Johann;Mantalaris, Athanasios
通讯作者：
Mantalaris, Athanasios
影响因子：
3.8
作者：
Glicklis, R;Merchuk, JC;Cohen, S
通讯作者：
Cohen, S