PROLONGATION OF CARDIAC XENOGRAFT SURVIVAL BY DEPLETION OF COMPLEMENT
PROLONGATION OF CARDIAC XENOGRAFT SURVIVAL BY DEPLETION OF COMPLEMENT
复制标题
DOI：
10.1097/00007890-199304000-00033
复制
发表时间：
1993-04-01
期刊：
影响因子：
6.2
通讯作者：
BAUMGARTNER
中科院分区：
文献类型：
--
作者：
LEVENTHAL, JR;DALMASSO, AP;BAUMGARTNER
补体（C）活化被认为是异种移植物超急性排斥反应的关键。我们通过研究C耗竭的受体的结果，使用高度纯化形式的眼镜蛇毒因子（CVF），（豚鼠[GP]-大鼠）和大（猪对狒狒）对大鼠静脉注射30或60单位CVF单次剂量可完全消除溶血C活性长达72小时。与几乎检测不到的血清C3水平相关。每天30 U/kg或每隔一天60 U/kg的剂量持续7天，维持C耗竭，无发病率或死亡率。在CVF治疗期间接受GP心脏异种移植的大鼠显著延长了异种移植物的存活时间（CVF治疗组大鼠为88 ± 10小时，对照组大鼠为18.6 ± 7.2分钟，P
Complement (C) activation is thought to be critical for the hyperacute rejection of xenografts. We investigated the role of C in the rejection of discordant cardiac xenografts by studying outcome in recipients depleted of C, using a highly purified form of cobra venom factor (CVF) in both a small (guinea pig [GP]-to-rat) and large (pig-to-baboon) animal model.A single dose of 30 or 60 units CVF given i.v. to rats completely abrogated hemolytic C activity for up to 72 hr. The lack of hemolytic C activity correlated with nearly undetectable serum levels of C3. Doses of 30 U/kg daily or 60 U/kg every other day over a 7-day period sustained C depletion without morbidity or mortality. Rats receiving GP cardiac xenografts during CVF therapy had significantly prolonged xenograft survival (88 +/- 10 hr in CVF-treated rats vs. 18.6+/-7.2 min in control rats, P