A genomic analysis of Drosophila somatic sexual differentiation and its regulation
A genomic analysis of Drosophila somatic sexual differentiation and its regulation
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DOI：
10.1242/dev.01077
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发表时间：
2004-05-01
期刊：
影响因子：
4.6
通讯作者：
Baker, BS
中科院分区：
文献类型：
--
作者：
Arbeitman, MN;Fleming, AA;Baker, BS
在几乎所有的动物中，雄性和雌性在形态、生理和行为上都是不同的。使用代表果蝇基因的三分之一的cDNA微阵列来鉴定在体细胞组织中性别差异表达的基因，我们对成年雄性和雌性进行了表达分析：（1）野生型;（2）缺乏生殖系;或（3）性别决定调控基因的突变体。统计学分析鉴定了63个基因在索马体中的性别差异表达，其中20个基因已被RNA印迹证实。其中11个基因的原位杂交实验表明，它们仅在内生殖器官中存在性别差异表达。这些基因编码的产物的性质提供了对这些生殖组织的分子生理学的深入了解。对这些基因的调控分析表明，它们的成年表达模式是由发育过程中的性别层次结构所指定的，并且doubletex可能以不同的方式发挥作用来设置它们的活动。
In virtually all animals, males and females are morphologically, physiologically and behaviorally distinct. Using cDNA microarrays representing one-third of Drosophila genes to identify genes expressed sex-differentially in somatic tissues, we performed an expression analysis on adult males and females that: (1) were wild type; (2) lacked a germline; or (3) were mutant for sex-determination regulatory genes. Statistical analysis identified 63 genes sex-differentially expressed in the soma, 20 of which have been confirmed by RNA blots thus far. In situ hybridization experiments with 11 of these genes showed they were sex-differentially expressed only in internal genital organs. The nature of the products these genes encode provides insight into the molecular physiology of these reproductive tissues. Analysis of the regulation of these genes revealed that their adult expression patterns are specified by the sex hierarchy during development, and that doublesex probably functions in diverse ways to set their activities.