In vitro mineralisation of chitosan membranes carrying phosphate functionalities
In vitro mineralisation of chitosan membranes carrying phosphate functionalities
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DOI：
10.4028/www.scientific.net/kem.254-256.577
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发表时间：
2004-01-01
期刊：
影响因子：
--
通讯作者：
Barbosa, MA
中科院分区：
文献类型：
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作者：
Amaral, IF;Granja, PL;Barbosa, MA
采用H3PO4/P2O5/Et3PO4/丁醇法对鱿鱼壳聚糖膜进行磷酸化。将p -壳聚糖膜浸泡在Ca(OH)(2)或NaOH溶液中，分别得到Na盐或Ca盐。研究了这些材料在模拟生理条件下成核磷酸钙的能力。SEM-EDS研究表明，在Ca(OH)(2)溶液中孵育后，p -壳聚糖膜表面存在一层磷酸钙矿物层。在碱性条件下，由于溶液中的钙离子的螯合作用，离子结合磷酸盐功能的释放可能有助于磷酸钙前体位点的形成。EDS和ATR-FTIR分析表明，在模拟体液（SBF）中浸泡期间，这些膜表面形成了由碳酸化磷灰石组成的多层多孔矿物结构。未经改性的膜和在NaOH溶液中预孵育的p -壳聚糖膜没有矿化。
Squid chitosan membranes were phosphorylated through the H3PO4/P2O5/Et3PO4/butanol method. P-chitosan membranes were immersed in Ca(OH)(2) or NaOH solutions, in order to obtain the Na or the Ca salts, respectively. These materials were investigated regarding their ability to nucleate calcium phosphates, under simulated physiologic conditions. SEM-EDS studies revealed the presence of a calcium phosphate mineral layer all over the surface of P-chitosan membranes, after incubation in Ca(OH)(2) solution. The release of ionically bound phosphate functionalities under alkaline conditions, possibly contributed to the formation of calcium phosphate precursor sites, due to the chelation of calcium ions from solution. During the immersion in Simulated Body Fluid (SBF), a multilayer porous mineral structure composed of poorly crystalline carbonated apatite was formed on the surface of these membranes, as shown by EDS and ATR-FTIR analysis. Unmodified membranes and P-chitosan membranes pre-incubated in NaOH solution did not mineralise.