Monolayered mesenchymal stem cells repair scarred myocardium after myocardial infarction
Monolayered mesenchymal stem cells repair scarred myocardium after myocardial infarction
复制标题
DOI：
10.1038/nm1391
复制
发表时间：
2006-04-01
期刊：
影响因子：
82.9
通讯作者：
Mori, H
中科院分区：
文献类型：
--
作者：
Miyahara, Y;Nagaya, N;Mori, H
间充质干细胞是一种多能细胞，可以分化为心肌细胞和血管内皮细胞。在这里，我们显示，使用细胞片技术，单层间充质干细胞移植到梗死大鼠心脏后具有多能性和自我繁殖的特性。我们培养脂肪组织来源的间充质干细胞，其特征在于使用温度响应培养皿的流式细胞仪。冠脉结扎后四周，我们将单层间充质干细胞移植到瘢痕心肌上。移植后，移植片逐渐生长，形成一个厚厚的层，包括新形成的血管，未分化的细胞和少数心肌细胞。间充质干细胞片层还通过旁分泌途径触发血管生成。与成纤维细胞片不同，单层间充质干细胞逆转了瘢痕区域的壁变薄，并改善了心肌梗死大鼠的心脏功能。因此，单层间充质干细胞移植可能成为心脏组织再生的一种新的治疗策略。
Mesenchymal stem cells are multipotent cells that can differentiate into cardiomyocytes and vascular endothelial cells. Here we show, using cell sheet technology, that monolayered mesenchymal stem cells have multipotent and self-propagating properties after transplantation into infarcted rat hearts. We cultured adipose tissue-derived mesenchymal stem cells characterized by flow cytometry using temperature-responsive culture dishes. Four weeks after coronary ligation, we transplanted the monolayered mesenchymal stem cells onto the scarred myocardium. After transplantation, the engrafted sheet gradually grew to form a thick stratum that included newly formed vessels, undifferentiated cells and few cardiomyocytes. The mesenchymal stem cell sheet also acted through paracrine pathways to trigger angiogenesis. Unlike a fibroblast cell sheet, the monolayered mesenchymal stem cells reversed wall thinning in the scar area and improved cardiac function in rats with myocardial infarction. Thus, transplantation of monolayered mesenchymal stem cells may be a new therapeutic strategy for cardiac tissue regeneration.