Rapid structural elucidation of composite bacterial hopanoids by atmospheric pressure chemical ionisation liquid chromatography/ion trap mass spectrometry
Rapid structural elucidation of composite bacterial hopanoids by atmospheric pressure chemical ionisation liquid chromatography/ion trap mass spectrometry
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DOI：
10.1002/rcm.2911
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发表时间：
2007-01-01
影响因子：
2
通讯作者：
Farrimond, Paul
中科院分区：
文献类型：
--
作者：
Talbot, Helen M.;Rohmer, Michel;Farrimond, Paul
细菌藿烷多元醇（BHP）是由多种真细菌产生的膜脂质。然而，它们作为细菌种群和过程的分子标记物的使用直到最近才受到缺乏合适的快速方法来在复杂的环境基质中对其组成进行指纹分析的阻碍。采用离子阱质谱的新分析程序现在使我们能够调查不同生物和环境样品中BHP的发生情况，包括细菌培养物，土壤，以及最近和古老的沉积物。在这里，我们描述了使用大气压化学电离液相色谱/离子阱质谱法（APCI-LC/MSn）的一些以前确定的，但不太常见的BHP，如adenosylhopane和ribonylhopane的结构表征。本文所述的许多结构以前仅在一种或仅少量培养的生物体中报道过，所述培养的生物体已从大量细胞团（4-26 g）中分离并在纯化单个化合物后通过核磁共振（NMR）技术鉴定。现在，已经建立了特征性APCl片段化模式，可以仅使用少量材料（<50 mg）以及环境样品快速筛选更多的细菌培养物以获得这些非典型结构和快速增长的一套新结构。版权所有（c）2007约翰威利父子有限公司。
Bacteriohopanepolyols (BHPs) are membrane lipids produced by a wide range of eubacteria. Their use, however, as molecular markers of bacterial populations and processes has until recently been hampered by the lack of a suitable rapid method for fingerprinting their composition in complex environmental matrices. New analytical procedures employing ion trap mass spectrometry now allow us to investigate the occurrence of BHPs in diverse biological and environmental samples including bacterial cultures, soils, and recent and ancient sediments. Here, we describe the structural characterisation using atmospheric pressure chemical ionisation liquid chromatography/ion trap mass spectrometry (APCI-LC/MSn) of a number of previously identified but less commonly occurring BHPs such as adenosylhopane and ribonylhopane. Many of the structures described here have previously only been reported in one or just a small number of cultured organisms having been isolated from large amounts of cellular mass (4-26 g) and identified by nuclear magnetic resonance (NMR) techniques after purification of individual compounds. Now, having established characteristic APCl fragmentation patterns, it is possible to rapidly screen many more bacterial cultures using only small amounts of material (< 50mg) as well as environmental samples for these atypical structures and a rapidly growing suite of novel structures. Copyright (c) 2007 John Wiley & Sons, Ltd.