STE12, A PROTEIN INVOLVED IN CELL-TYPE-SPECIFIC TRANSCRIPTION AND SIGNAL TRANSDUCTION IN YEAST, IS PART OF PROTEIN DNA COMPLEXES
STE12, A PROTEIN INVOLVED IN CELL-TYPE-SPECIFIC TRANSCRIPTION AND SIGNAL TRANSDUCTION IN YEAST, IS PART OF PROTEIN DNA COMPLEXES
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DOI：
10.1101/gad.3.9.1349
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发表时间：
1989-09-01
影响因子：
10.5
通讯作者：
AMMERER, G
中科院分区：
文献类型：
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作者：
ERREDE, B;AMMERER, G
酿酒酵母的STE12基因对于交配所需基因的表达至关重要，例如那些参与信息素反应的基因，以及与交配无关但受转座因子Ty1相邻拷贝调节的基因。我们发现STE12蛋白是特定dna -蛋白复合物的一个组成部分，该复合物与来自Ty1和.alpha的转录控制元件形成。-信息素受体基因STE2尽管在这两个复合物中都有信息素依赖的转录激活序列受到保护，但竞争实验表明，这两个复合物在本质上是不同的。我们发现另一个参与细胞类型特异性转录的因子PRTF/GRM是STE2片段复合体的一个组成部分，而不是Ty1片段。我们提出STE12产物在不同的序列背景下与不同的转录因子相互作用，PRTF/GRM是这些因子之一。
The STE12 gene of Saccharomyces cerevisiae is essential for the expression of genes required for mating, such as those involved in pheromone response, and for genes unrelated to mating but regulated by the presence of an adjacent copy of the transposable element Ty1. We show that the STE12 protein is a component of specific DNA-protein complexes that form with transcriptional control elements from Ty1 and the .alpha.-pheromone receptor gene STE2. Although a sequence involved in pheromone-dependent transcriptional activation is protected in both complexes, competition experiments indicate that the complexes are intrinsically different from each other. We show that another factor involved in cell-type-specific transcription, PRTF/GRM, is a component of the complex with the STE2 fragment but not the Ty1 fragment. We propose that the STE12 product interacts with different transcription factors in different sequence contexts and that PRTF/GRM is one of these factors.