Interference of anaesthetics with radioligand binding in neuroreceptor studies
Interference of anaesthetics with radioligand binding in neuroreceptor studies
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DOI：
10.1007/s00259-003-1171-8
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发表时间：
2003-06-01
影响因子：
9.1
通讯作者：
Knudsen, GM
中科院分区：
文献类型：
--
作者：
Elfving, B;Bjornholm, B;Knudsen, GM
新的发射断层成像配体的评估通常在动物身上进行。为了使动物在扫描过程中处于受限的位置，麻醉几乎是不可避免的。在离体大鼠实验中，我们分别用[H-3]-(S)-西酞普兰、[F-18]阿坦色林和[I-125]PE2I研究了氯胺酮/噻嗪、唑来替莱混合物、异氟醚和氟烷对血清素再摄取位点、血清素（2A）受体和多巴胺再摄取位点的干扰。氯胺酮/噻嗪使[H-3]-(S)-西酞普兰的靶本比（平均+/- SD）从1.5+/-0.19降至0.81+/-0.19 (P
Evaluations of new emission tomography ligands are usually carried out in animals. In order to keep the animals in a restricted position during the scan session, anaesthesia is almost inevitable. In ex vivo rat studies we investigated the interference of ketamine/xylazine, zoletile mixture, isoflurane and halothane with the serotonin re-uptake site, the serotonin(2A) receptor and the dopamine re-uptake site by use of [H-3]-(S)-citalopram, [F-18]altanserin and [I-125]PE2I, respectively. Ketamine/xylazine decreased the target-to-background ratio (mean +/- SD) of [H-3]-(S)-citalopram from 1.5+/-0.19 to 0.81+/-0.19 (P