Efficient quenching of oligomeric fluorophores on a DNA backbone
Efficient quenching of oligomeric fluorophores on a DNA backbone
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DOI：
10.1021/ja075968a
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发表时间：
2007-12-19
影响因子：
15
通讯作者：
Kool, Eric T.
中科院分区：
文献类型：
--
作者：
Wilson, James N.;Teo, Yin Nah;Kool, Eric T.
描述了一系列含荧光基团“碱”的低聚脱氧核苷的猝灭特性。在吸收光谱和发射光谱中，相邻的、圆周率堆积的果核序列显示出比由介入腺嘌呤隔绝的果核更强的电子相互作用。N，N‘-二甲基-4，4’-联吡啶对激基缔合物和激基缔合物的形成是有效的猝灭方法，其Stern-Volmer常数可与共轭聚合物的“超猝灭”方案相媲美。
The quenching properties of a series of oligodeoxyribosides bearing fluorophore "bases" are described. Sequences of adjacent, pi-stacked pyrenes exhibit stronger electronic interactions visible in both absorbance and emission spectra than pyrenes that are insulated by intervening adenines. Quenching by N,N'-dimethyl-4,4'-bipyridinium dichloride is efficient for excimer- and exciplex-forming oligomers, with Stern-Volmer constants comparable to conjugated polymer "superquenching" schemes.