Aqueous extract of peanut skin and its main constituent procyanidin A1 suppress serum IgE and IgG1 levels in mice-immunized with ovalbumin.
Aqueous extract of peanut skin and its main constituent procyanidin A1 suppress serum IgE and IgG1 levels in mice-immunized with ovalbumin.
复制标题
花生皮的水提取物及其主要成分原花青素 A1 可抑制卵清蛋白免疫小鼠的血清 IgE 和 IgG1 水平。
DOI：
--
复制
发表时间：
2007
影响因子：
2
通讯作者：
T. Ohta
中科院分区：
文献类型：
--
作者：
F. Takano;T. Takata;A. Yoshihara;Yuka Nakamura;Y. Arima;T. Ohta
在本研究中，我们研究了花生的一种水提物(Arachim subgaea L.)种子皮(PSE)及其主要成分原花青素A1(PA)对变应原卵白蛋白(OVA)过敏反应的影响。经腹膜腔注射OVA免疫的小鼠血清中抗OVA IgE和总IgG1水平显著高于未经治疗的对照组小鼠。口服PSE 10~100 mg/kg/d(连续21天)可降低OVA免疫后第21天的抗OVA、IgE和IgG1水平。这种PSE还抑制了OVA诱导的脾重量增加和外周血白细胞计数的增加。苹果(30 mg/kg)和葡萄籽(30 mg/kg)的多酚富集物也降低了抗OVA IgE水平，但不影响总IgG1水平。口服从PSE中提纯的PA(1~10 mg/kg/d)可抑制小鼠血清中的IgE和总IgG1水平。所有受试样品均能降低OVA免疫小鼠血清IL-4水平的升高，而PSE和PA是唯一能逆转OVA降低的干扰素-γ水平的化合物。这些结果表明，摄入PSE或其主要活性成分PA可能通过抑制免疫球蛋白的合成来预防过敏反应，其作用机制部分是由于PSE和PA对T辅助细胞因子产生的调节。
In this study, we investigated the effects of an aqueous extract of peanut (Arachis hypogaea L.) seed skin (PSE) and its main constituent procyanidin A1 (PA) on the allergic response to allergen ovalbumin (OVA) in a mouse model. Mice immunized interaperitoneally with OVA dramatically increased anti-OVA IgE and total IgG1 levels in serum compared with non-treated control mice. Oral injection of PSE at doses ranging from 10 to 100 mg/kg/d (for 21 consecutive days) decreased anti-OVA IgE and IgG1 levels 21 d after OVA-immunization. OVA-induced increments in spleen weight and peripheral white blood cell count were also suppressed by this PSE administration. Polyphenol-enriched fractions from apple (30 mg/kg) and grape seed (30 mg/kg) also decreased anti-OVA IgE level but did not affect total IgG1 levels. Oral injection of PA (1 to 10 mg/kg/d) purified from PSE resulted in a suppression of IgE and total IgG1 levels in serum. An increment of serum interleukin-4 level in mice that were immunized with OVA was reduced by all tested samples, whereas PSE and PA were the only compounds that could reverse the reduced interferon-gamma level by OVA. These findings suggest that intake of PSE or its main active constituent PA may prevent an allergic reaction by inhibiting immunoglobulin synthesis, and the mechanism of this action of PSE and PA is in part due to their regulation of T helper cytokine production.
影响因子：
4.4
作者：
G. Lack;K. Bradley;Eckard Hamelmann;H. Renz;J. Loader;D. Leung;G. Larsen;E. Gelfand
通讯作者：
G. Lack;K. Bradley;Eckard Hamelmann;H. Renz;J. Loader;D. Leung;G. Larsen;E. Gelfand
影响因子：
4.7
作者：
Jifu Zhao;Jiannong Wang;Yingjie Chen;R. Agarwal
通讯作者：
Jifu Zhao;Jiannong Wang;Yingjie Chen;R. Agarwal