A six-month audit of the isolation of Fusobacterium necrophorum from patients with sore throat in a district general hospital
A six-month audit of the isolation of Fusobacterium necrophorum from patients with sore throat in a district general hospital
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DOI：
10.1080/09674845.2007.11732757
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发表时间：
2007-01-01
影响因子：
1.9
通讯作者：
Dytrych, J. K.
中科院分区：
文献类型：
--
作者：
Amess, J. A.;O'Neill, W.;Dytrych, J. K.
坏死梭杆菌是一种专性厌氧菌，被认为是人类口腔和泌尿生殖道正常植物群的成员。它与深层感染有关，并于1936年由法国微生物学家Lemierre首次描述。现在有强有力的证据表明，它也是复发性咽喉痛和持续性咽喉痛综合征（PSTS）的原因，而不会导致全身感染。它被认为是第二个最常见的原因咽喉痛后，A组P-溶血性链球菌。本研究在伊斯特本地区综合医院进行，为期6个月（2004年10月至2005年3月）。对实验室收到的所有咽拭子进行常规培养，以检测溶血性A组链球菌和致病性棒状杆菌。在研究期间，接种含有新霉素的额外苛养厌氧血琼脂平板，在第二条和第三条条纹的交界处放置30 μ g万古霉素圆盘。48 h后检查是否存在坏死梭菌。研究期间共处理了1157份拭子：156份为A组溶血性链球菌阳性，57份为坏死梭菌阳性，47份为C组溶血性链球菌阳性，9份为G组溶血性链球菌阳性，1份为C。溃疡。患者年龄范围从不到1岁到88岁。11-25岁年龄组患者占绝大多数，分离率为9.48%（44/464）。该年龄组占研究期间收到的拭子的40%（464/1157），其中77%（44/57）为坏死梭菌阳性。A组溶血性链球菌的总分离率为13.5%，在0-10岁和26-35岁年龄段的分离率最高，为23%。在本研究中，这两种微生物共占所有咽喉感染的18.0%（213/1157）。结果表明，F坏死是第二组A型溶血性链球菌作为咽喉痛的原因，特别是在年轻的成年人，并应考虑引进常规培养。
Fusobacterium necrophorum is an obligate anaerobe believed to be a member of the normal flora of the human oropharangeal and urogenital tract. It has been associated with deep-seated infections and was first described in 1936 by Lemierre, a French microbiologist. There is now strong evidence to suggest that it is also a cause of recurrent sore throat and persistent sore throat syndrome (PSTS) without leading to full systemic infection. It is considered to be the second most common cause of sore throat after group A P-haemolytic streptococci. This study was performed over a six-month period (October 2004 to March 2005) at the Eastbourne District General Hospital. All throat swabs received in the laboratory are cultured routinely for haemolytic group A streptococci and pathogenic Corynebacteria spp. During the study period an extra fastidious anaerobic blood agar plate with neomycin was inoculated, with a 30 mu g vancomycin disc placed at the junction of the second and third streaks. This was examined after 48 h for the presence of F necrophorum. A total of 1157 swabs were processed during the study period: 156 were positive for haemolytic group A streptococci, 57 were positive for F necrophorum, 47 for group C haemolytic streptococci, nine for group G haemolytic streptococci, and one was positive for C. ulcerans. Patient age ranged from less than a year old to 88. The majority of F necrophorum isolates were from patients in the 11-25 age group, with an isolation rate of 9.48% (44/464). This age group accounted for 40% (464/1157) of the swabs received during the study period and 77% (44/57) of these were positive for F necrophorum. Group A haemolytic streptococci showed an overall isolation rate of 13.5%, with peaks of 23% in the 0-10 and 26-35 age ranges. Together, these two organisms were responsible for 18.0% (213/1157) of all throat infections in this study. The results presented here indicate that F necrophorum is second to group A haemolytic streptococci as a cause of sore throat, especially in the young adult, and introduction of routine culture should considered.