Secretion of chemokines and other cytokines in allergen-induced nasal responses: inhibition by topical steroid treatment.
Secretion of chemokines and other cytokines in allergen-induced nasal responses: inhibition by topical steroid treatment.
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过敏原诱导的鼻反应中趋化因子和其他细胞因子的分泌：局部类固醇治疗的抑制。
DOI：
10.1164/ajrccm.152.3.7545059
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Alam,R
中科院分区：
文献类型：
--
作者：
Sim,TC;Reece,LM;Hilsmeier,KA;Grant,JA;Alam,R
我们已经证明了抗原攻击后鼻分泌物中促过敏细胞因子的检测。我们的目的是确定过敏原激发后趋化因子（白细胞介素[IL]-8、巨噬细胞炎症蛋白-1 α [MIP-1 α]和RANTES）和其他细胞因子（IL-1 β和粒细胞/巨噬细胞集落刺激因子[GM-CSF]）的分泌动力学及其类固醇治疗的抑制作用。10名过敏患者以双盲、随机、交叉的方式接受二丙酸倍氯米松（BDP）或安慰剂治疗。治疗1周后进行变应原激发试验。鼻分泌物连续收集过敏原攻击后11小时，通过矩阵法。受试者在鼻分泌物恢复的每个时间点保持症状评分。通过特异性酶联免疫吸附试验测定细胞因子。在BDP治疗期间，早期（ER）和/或晚期反应（LPR）期间每种细胞因子的平均峰值和总症状评分显著降低（p < 0.05）。ER（IL-1 β和MIP-1 α）和LPR（所有细胞因子）期间细胞因子水平与相应的总症状评分相关（p < 0.05）。我们的研究结果记录了过敏原激发后鼻分泌物中IL-1 β、GM-CSF和趋化因子的局部升高以及类固醇对其的抑制。我们推测抑制鼻粘膜细胞因子的产生和分泌可能有助于局部类固醇的临床疗效。
We have demonstrated the detection of proallergic cytokines in the nasal secretions after antigen challenges. Our aim was to determine the secretion kinetics of chemokines (interleukin [IL]-8, macrophage inflammatory protein-1 alpha [MIP-1 alpha], and RANTES) and other cytokines (IL-1 beta and granulocyte/macrophage colony-stimulating factor [GM-CSF] after allergen challenges and their inhibition by steroid therapy. Ten allergic patients were given either beclomethasone dipropionate (BDP) or placebo in a double-blind, randomized, crossover manner. Allergen challenges were performed after 1 wk of treatment. Nasal secretions were collected serially for 11 h after allergen challenge by a matrix method. Subjects maintained symptom scores at each time point of nasal secretion recovery. Cytokines were measured by specific enzyme-linked immunosorbent assays. The mean peak values for each cytokine and total symptom scores during the early (ER) and/or late-phase reactions (LPR) were significantly reduced during the BDP treatment period (p < 0.05). The levels of cytokine correlated (p < 0.05) with corresponding total symptom scores during ER (IL-1 beta and MIP-1 alpha) and LPR (all cytokines). Our findings document local elevations of IL-1 beta, GM-CSF, and chemokines in the nasal secretions after allergen challenges and their inhibition by steroids. We speculate that the inhibition of cytokine production and secretion in the nasal mucosa may contribute to the clinical efficacy of topical steroids.