Miniaturized capillary electrophoresis system with ultraviolet photometric detection combined with flow injection sample introduction using a modified falling-drop interface
Miniaturized capillary electrophoresis system with ultraviolet photometric detection combined with flow injection sample introduction using a modified falling-drop interface
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DOI：
10.1016/j.aca.2004.03.028
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发表时间：
2004-06-25
影响因子：
6.2
通讯作者：
Fang, ZL
中科院分区：
文献类型：
--
作者：
Cao, XD;Fang, Q;Fang, ZL
为了实现高通量连续进样，将带有紫外-可见检测的小型化毛细管电泳联用到流动注射(FI)系统中。对商用毛细管电泳仪的盒进行了改装，以容纳6.5厘米长的二氧化硅毛细管和一个流过的废物池。试剂盒被插入商用紫外光检测器的流动小室，光束聚焦在毛细管上，并由试剂盒上的两个球透镜收集。毛细管入口位于盒和检测器外，位于垂直3.5 mm的顶部。直径的玻璃棒，与电极紧密接触。通过FI系统注入的样品自由地落在柱子的顶部，覆盖了毛细管进口和电极。在不间断分离电压下实现了CE连续进样，分离电压通过液滴的不连续而与FI系统隔离。建立了磺胺甲恶唑(SMZ)和甲氧苄氨嘧啶(TMP)的快速分离界面和紫外检测系统，实现了磺胺甲恶唑(SMZ)和甲氧苄啶(TMP)的快速分离，高通量大于48h(-1)，低残留为2%。分离效率为8微米，检出限分别为1.0mg1(-1)和0.5mg1(-1)(3sigma)。(C)2004爱思唯尔B.V.保留所有权利。
A miniaturized capillary electrophoresis (CE) system with UV-Vis detection was coupled to a flow injection (FI) system for achieving high throughput continuous sample introduction. The cassette of a commercial CE instrument was modified to hold a 6.5 cm long silica capillary and a flow-through waste reservoir. The cassette was inserted into the flow-cell chamber of a commercial UV detector, with the light beam focused on the capillary and collected by two ball lenses on the cassette. The capillary inlet, left outside the cassette and detector, was positioned on the top of a vertical 3.5 mm. diameter glass rod, in close contact with an electrode. Samples injected through the FI system dropped freely on top of the pillar, covering the capillary inlet and electrode. Continuous sample introduction was achieved for CE separations under non-interrupted separation voltage, which was isolated from the FI system through the discontinuity of droplets. The newly developed interface and UV detection system was used for fast separation of sulphamethoxazole (SMZ) and trimethoprim (TMP) in sulphatrim tablets, achieving a high throughput of over 48 h(-1), and a low carryover of 2%. Separation efficiencies of 8 mum plate height and detection limits of 1.0mg 1(-1) for SMZ and 0.5 mg 1(-1) (3sigma) for TMP were obtained. (C) 2004 Elsevier B.V. All rights reserved.