Layer-by-layer self-assembly of hollow dextran sulfate/chitosan-coated zein nanoparticles loaded with crocin: Fabrication, structural characterization and potential biological fate
Layer-by-layer self-assembly of hollow dextran sulfate/chitosan-coated zein nanoparticles loaded with crocin: Fabrication, structural characterization and potential biological fate
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负载藏红花素的空心硫酸葡聚糖/壳聚糖包被的玉米醇溶蛋白纳米粒子的层层自组装：制造、结构表征和潜在的生物命运
DOI：
10.1016/j.foodhyd.2021.107420
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发表时间：
2021-12-03
影响因子：
10.7
通讯作者：
Zheng, Fuping
中科院分区：
文献类型：
--
作者：
Song, Gongshuai;Liu, Jiayuan;Zheng, Fuping
藏红花素是一种水溶性类胡萝卜素，已显示出改善阿尔茨海默病（AD）等神经退行性疾病的潜力。然而，由于其高度不稳定性以及对加工条件敏感，其应用受到限制。在此，以碳酸钠为牺牲模板，通过层层（LbL）自组装技术制备了负载藏红花素的中空硫酸葡聚糖/壳聚糖（DS/CH）包覆玉米醇溶蛋白纳米颗粒（NPs）。平均粒径、多分散指数（PDI）、包封率、zeta电位、形态（如透射电子显微镜（TEM）和原子力显微镜（AFM））以及傅里叶变换红外光谱（FTIR）观察表明，负载藏红花素的LbL中空玉米醇溶蛋白纳米颗粒的表面是通过聚电解质通过静电相互作用、氢键和疏水相互作用沉积而形成的。体外模拟胃肠消化和抗氧化活性测定表明，与分散在水中的未包覆负载藏红花素的玉米醇溶蛋白纳米颗粒相比，这些纳米颗粒表现出更好的控释行为和更强的抗氧化活性。此外，对纳米颗粒对AD细胞模型潜在影响的研究表明，分化的SH - SY5Y细胞的β - 淀粉样蛋白（Aβ1 - 42）浓度从300 pg/mg降至170 pg/mg。这些结果表明，负载藏红花素的中空DS/CH包覆玉米醇溶蛋白纳米颗粒的LbL自组装可开发成为一种有前景的用于AD有效治疗方法的递送系统，并为递送生物活性成分提供了一种新的实用技术。
Crocin is a water-soluble carotenoid and has shown potential to improve the neurodegenerative disease such as Alzheimer's disease (AD). However, its application is limited due to the high instability and susceptibility to process conditions. Here, hollow dextran sulfate/chitosan (DS/CH)-coated zein nanoparticles (NPs) loaded with crocin using sodium carbonate as a sacrificing template were fabricated by a layer-by-layer (LbL) self-assembly technique. The mean particle size, polydispersity index (PDI), encapsulation efficiency, zeta potential, morphology (e.g. TEM and AFM), and FTIR observation revealed that the surface of crocin-loaded LbL hollow zein NPs was generated by the deposition of polyelectrolyte through electrostatic interactions, hydrogen bonding, and hydrophobic interactions. In vitro simulated gastrointestinal digestion and antioxidant activity assays indicated that the NPs exhibited better controlled release behavior and stronger antioxidant activity than that of uncoated crocin-loaded zein NPs dispersed in water. Moreover, the study of the potential effect of the NPs on AD cell model showed that the amyloid-beta (A beta 1-42) concentration of differentiated SH-SY5Y cells decreased from 300 pg/mg to 170 pg/mg. These results reveal that LbL self-assembly of hollow DS/CH-coated zein NPs loaded with crocin can be developed as a promising delivery system for effective therapeutic approach of AD, and provide a new practical technique for delivering bioactive ingredients.