Detailed analysis at a single-cell level of cells undergoing pancreatic differentiation.
Detailed analysis at a single-cell level of cells undergoing pancreatic differentiation.
复制标题
对正在进行胰腺分化的细胞进行单细胞水平的详细分析。
DOI：
10.1111/jdi.13140
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Kume S.
中科院分区：
文献类型：
--
作者：
Shiraki S;Kume S.
多能干细胞（PSC）来源的b细胞有望成为1型糖尿病患者细胞替代治疗的来源。最近有几个方案描述了胰腺b细胞的产生。道格拉斯梅尔顿实验室的最新报告充分利用单细胞核糖核酸（RNA）测序（scRNA-seq），提供了干细胞胰腺分化的深入知识。1型糖尿病是一种糖尿病，其中胰腺b细胞产生很少或没有胰岛素。胰腺或胰岛移植提供了有效的治疗方法，可维持正常血糖数年，并减少并发症的进展。因此，供体数量有限，需要替代细胞，PSC衍生的b细胞有望成为1型糖尿病患者细胞替代治疗的有吸引力的来源。最近已经报道了从PSC产生胰腺b细胞的几种方案2，3，但是为了进一步提高分化效率和成熟度，有必要表征分化过程中出现的单个细胞。到目前为止，几个小组已经报道了分化的大量细胞群体的转录组分析，但没有研究在单细胞水平上全面确定胰腺分化的每个阶段的所有细胞类型的身份和状态。
Pluripotent stem cells (PSCs)-derived bcells are expected to be a source for cell replacement therapy of type 1 diabetes mellitus patients. Several protocols have recently described the generation of pancreatic b-cells. The latest report from the Douglas Melton laboratory, fully utilizing single-cell ribonucleic acid (RNA) sequencing (scRNA-seq), provides indepth knowledge of stem cell pancreatic differentiation1.Type 1 diabetes mellitus is a form of diabetes in which very little or no insulin is produced by the pancreatic b-cells. Pancreas or islet transplantation provides effective cures for maintaining normoglycemia for several years, and reduces the progression of complications. The limited number of donors, therefore, calls for alternative cells, and PSCsderived b-cells are expected to be an attractive source for cell replacement therapy of type 1 diabetes mellitus patients. Several protocols have recently been reported for the generation of pancreatic b-cells from PSCs2, 3, but to further increase differentiation efficiency and maturity, it is necessary to characterize the individual cells that appear during differentiation. To date, several groups have reported on transcriptome analysis of differentiating bulk cell populations, but no research has comprehensively determined the identity and states of all the cell types at each stage of pancreatic differentiation at a single-cell level.