A controlled release of antibiotics from calcium phosphate-coated poly(lactic-co-glycolic acid) particles and their in vitro efficacy against Staphylococcus aureus biofilm
A controlled release of antibiotics from calcium phosphate-coated poly(lactic-co-glycolic acid) particles and their in vitro efficacy against Staphylococcus aureus biofilm
复制标题
DOI：
10.1007/s10856-013-5125-9
复制
发表时间：
2014-03-01
影响因子：
3.7
通讯作者：
Loo, Say Chye Joachim
中科院分区：
文献类型：
--
作者：
Bastari, Kelsen;Arshath, Mohamed;Loo, Say Chye Joachim
陶瓷-聚合物杂化颗粒，用于骨髓炎治疗，通过制备聚（乳酸-共-乙醇酸）颗粒通过乳化溶剂蒸发技术，然后通过表面吸附-成核法的磷酸钙（CaP）涂层。通过扫描电子显微镜、能量色散X射线光谱和X射线光电子能谱证实了颗粒表面上存在CaP涂层。随后，用于治疗骨感染的两种抗生素，萘夫西林（亲水性）和左氧氟沙星（两亲性），装载到这些杂化粒子和它们的体外药物释放研究。CaP涂层显示出减少突释，同时提供抗生素的持续释放长达4周。针对金黄色葡萄球菌的体外细菌研究证明了这些负载抗生素的混合颗粒抑制生物膜形成以及恶化已建立的生物膜的能力，使得该混合系统成为进一步研究骨髓炎治疗的潜在候选者。
Ceramic-polymer hybrid particles, intended for osteomyelitis treatment, were fabricated by preparing poly(lactic-co-glycolic acid) particles through an emulsion solvent evaporation technique, followed by calcium phosphate (CaP) coating via a surface adsorption-nucleation method. The presence of CaP coating on the surface of the particles was confirmed by scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray photoelectron spectroscopy. Subsequently, two antibiotics for treating bone infection, nafcillin (hydrophilic) and levofloxacin (amphiphilic), were loaded into these hybrid particles and their in vitro drug release studies were investigated. The CaP coating was shown to reduce burst release, while providing sustained release of the antibiotics for up to 4 weeks. In vitro bacterial study against Staphylococcus aureus demonstrated the capability of these antibiotic-loaded hybrid particles to inhibit biofilm formation as well as deteriorate established biofilm, making this hybrid system a potential candidate for further investigation for osteomyelitis treatment.