KBTBD7, a novel human BTB-kelch protein, activates transcriptional activities of SRE and AP-1
KBTBD7, a novel human BTB-kelch protein, activates transcriptional activities of SRE and AP-1
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KBTBD7 是一种新型人类 BTB-kelch 蛋白，可激活 SRE 和 AP-1 的转录活性。
DOI：
10.5483/bmbrep.2010.43.1.017
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发表时间：
2010-01-31
期刊：
影响因子：
3.8
通讯作者：
Wu, Xiushan
中科院分区：
文献类型：
--
作者：
Hu, Junjian;Yuan, Wuzhou;Wu, Xiushan
本研究从人胚胎心脏cDNA文库中克隆了一个新的BTB-kelch蛋白，命名为KBTBD 7。KBTBD 7的cDNA长3,008 bp，编码684个氨基酸（77.2 kD）的蛋白质产物。这种蛋白质在不同物种的进化中高度保守。Western blot分析表明，一个77 kD的KBTBD 7特异性蛋白在所有胚胎组织中广泛表达。在COS-7细胞中，KBTBD 7蛋白定位于细胞质。KBTBD 7是一种转录激活因子，当融合到GAL 4 DNA结合结构域时。缺失分析表明BTB结构域和kelch重复序列是转录激活的主要区域。KBTBD 7在MCF-7细胞中的过表达激活了激活蛋白-1（AP-1）和血清反应元件（SRE）的转录活性，siRNA可以减轻这种激活。这些结果表明，KBTBD 7蛋白可能作为一个新的转录激活因子在丝裂原活化蛋白激酶（MAPK）信号。[BMB reports 2010; 43（1）：17-22]。
In this study, a novel member of BTB-kelch proteins, named KBTBD7, was cloned from a human embryonic heart cDNA library. The cDNA of KBTBD7 is 3,008 bp long and encodes a protein product of 684 amino acids (77.2 kD). This protein is highly conserved in evolution across different species. Western blot analysis indicates that a 77 kD protein specific for KBTBD7 is wildly expressed in all embryonic tissues examined. In COS-7 cells, KBTBD7 proteins are localized to the cytoplasm. KBTBD7 is a transcription activator when fused to GAL4 DNA-binding domain. Deletion analysis indicates that the BTB domain and kelch repeat motif are main regions for transcriptional activation. Overexpression of KBTBD7 in MCF-7 cells activates the transcriptional activities of activator protein-1 (AP-1) and serum response element (SRE), which can be relieved by siRNA. These results suggest that KBTBD7 proteins may act as a new transcriptional activator in mitogen-activated protein kinase (MAPK) signaling. [BMB reports 2010; 43(1): 17-22].