PRODUCTION OF MONOCLONAL-ANTIBODY TO A LATE INTRACELLULAR EPSTEIN-BARR VIRUS-INDUCED ANTIGEN
PRODUCTION OF MONOCLONAL-ANTIBODY TO A LATE INTRACELLULAR EPSTEIN-BARR VIRUS-INDUCED ANTIGEN
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DOI：
10.1016/0042-6822(84)90402-1
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发表时间：
1984-01-01
期刊：
影响因子：
3.7
通讯作者：
PEARSON, GR
中科院分区：
文献类型：
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作者：
KISHISHITA, M;LUKA, J;PEARSON, GR
制备了一种抗EB病毒(EBV)诱导的晚期胞内蛋白的单抗，命名为L2。通过免疫荧光检测，该蛋白在产生病毒的细胞中表达，但在EBV基因组阳性的非产生细胞系、EBV基因组阴性的细胞系或在膦乙酸存在下培养的产生细胞培养中不表达。该抗体不与感染细胞的膜发生反应，表明它不是针对EBV诱导的膜抗原成分。该单抗可识别相对分子质量为.apprx的糖蛋白。125K十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法。当从人类淋巴母细胞系P3HR-1细胞系中分离出这种糖蛋白时，一直被发现比从B-95-8细胞系中分离出的糖蛋白稍大一些。通过免疫亲和层析从两个细胞系中分离得到的该蛋白的肽图也发现了类似的差异。血清学研究表明，这种125K糖蛋白是免疫荧光法确定的病毒衣壳抗原(VCA)复合体的主要成分。
A monoclonal antibody designated L2 was produced against a late intracellular protein induced by Epstein-Barr virus (EBV). This protein was expressed in cells producing virus but not in EBV genome-positive nonproducer cell lines, EBV genome-negative cell lines, or producer cultures cultivated in the presence of phosphonoacetic acid as determined by immunofluorescence. The antibody did not react with the membranes of infected cells indicating that it was not directed against an EBV-induced membrane antigen component. The monoclonal antibody was shown to recognize a glycoprotein with a MW of .apprx. 125K by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. This glycoprotein was consistently found to be slightly larger when isolated from the human lymphoblastoid P3HR-1 cell line as opposed to the B-95-8 cell line. A similar difference was also noted by comparison of peptide maps of this protein isolated by immunoaffinity chromatography from the 2 cell lines. Serological studies indicated that this 125K glycoprotein was a major component of the viral capsid-antigen (VCA) complex as defined by immunofluorescence.