The role of cytochrome P450 2C3 in rabbit liver microsomal metabolism of 1-nitropyrene and 3-nitrofluoranthene.
The role of cytochrome P450 2C3 in rabbit liver microsomal metabolism of 1-nitropyrene and 3-nitrofluoranthene.
复制标题
细胞色素P450 2C3在兔肝微粒体1-硝基芘和3-硝基荧蒽代谢中的作用。
DOI：
10.1002/jbt.2570090204
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Koop,DR
中科院分区：
文献类型：
--
作者：
Howard,PC;McManus,ME;Koop,DR
研究了雄性家兔肝微粒体细胞色素P450对1 -硝基芘和3 -硝基氟蒽的代谢。在这项研究中，我们使用了兔P450 2C3特异性抑制抗体，并确定，在未经处理的雄性兔肝微粒体中，该抗体抑制了大约75%的细胞色素P450介导的1 -硝基芘和3 -硝基氟蒽的C -氧化。这些结果验证了我们之前的预测，即在未经处理的兔肝脏中，主要有一种细胞色素P450负责1‐硝基芘的微粒体代谢。
The male rabbit liver microsomal cytochrome P450 metabolism of 1‐nitropyrene and 3‐nitrofluoranthene was investigated. In this study, we used inhibitory antibodies specific for rabbit P450 2C3 and determined that, in untreated male rabbit liver microsomes, the antibody inhibited approximately 75% of the cytochrome P450‐mediated C‐oxidation of both 1‐nitropyrene and 3‐nitrofluoranthene. These results verify our previous prediction that mainly one cytochrome P450 is responsible for microsomal metabolism of 1‐nitropyrene in the untreated rabbit liver.