An efficient and biocompatible fluorescence resonance energy transfer system based on lanthanide-doped nanoparticles
An efficient and biocompatible fluorescence resonance energy transfer system based on lanthanide-doped nanoparticles
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DOI：
10.1088/0957-4484/21/45/455703
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发表时间：
2010-11-12
期刊：
影响因子：
3.5
通讯作者：
Sakka, Yoshio
中科院分区：
文献类型：
--
作者：
Di, Weihua;Li, Jie;Sakka, Yoshio
本工作展示了一种基于镧系元素掺杂的无机纳米粒子的高效且生物友好的荧光共振能量转移（FRET）系统。采用水溶液法合成了CePO（4）：Tb纳米棒，该纳米棒具有均匀的胶体分散体，在紫外光激发下发射明亮的绿色光，具有高的量子产率（约0.36）和长的荧光寿命（约3.50 ms）。当CePO（4）：Tb与罗丹明B（RhB）水溶液反应时，从Tb（3+）到RhB分子发生了有效的FRET，分别产生了良好分辨的供体和受体的比率发射，能量转移效率高达0.85。当与HeLa细胞在37 ℃孵育时，用RhB处理的CePO（4）：Tb显示明亮的细胞内发光，表明它可以成功地内化在细胞内，并且FRET保留在活细胞中。此外，细胞毒性测量表明我们目前的FRET系统具有良好的生物相容性和低细胞毒性。CePO（4）：Tb/RhB FRET体系具有供体量子产率高、能量转移效率高、荧光发射率高、生物相容性好等优点，表明该体系在生物事件监测方面具有很大的潜力。
This work demonstrates an efficient and bio-friendly fluorescence resonance energy transfer (FRET) system based on lanthanide-doped inorganic nanoparticles. A facile aqueous route was used to synthesize the CePO(4):Tb nanorods with homogeneous colloidal dispersion, which emits a bright green light with a high quantum yield (similar to 0.36) and a long fluorescence lifetime (similar to 3.50 ms) upon UV excitation. Upon treatment of CePO(4):Tb with aqueous Rhodamine B (RhB), an efficient FRET occurs from the Tb(3+) to the RhB molecules, giving rise to well resolved and ratiometric emissions of donors and acceptors, respectively, with an energy transfer efficiency of up to 0.85. When incubated with HeLa cells at 37 degrees C, the CePO(4):Tb treated with RhB shows bright intracellular luminescence, indicating that it can be successfully internalized inside the cells and the FRET remains in the living cells. Moreover, the cytotoxic measurements demonstrate good biocompatibility and low cytotoxicity of our present FRET system. The advantages presented above including high quantum yield of donors, high energy transfer efficiency, ratiometric fluorescent emission and good biocompatibility, indicate the high potential of the CePO(4):Tb/RhB FRET system for monitoring biological events.