The HIV lipidome:: A raft with an unusual composition
The HIV lipidome:: A raft with an unusual composition
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DOI：
10.1073/pnas.0511136103
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发表时间：
2006-02-21
影响因子：
11.1
通讯作者：
Kräusslich, HG
中科院分区：
文献类型：
--
作者：
Brügger, B;Glass, B;Kräusslich, HG
包膜病毒的脂质在病毒形态发生和感染性中发挥关键作用。它们来源于病毒出芽的宿主膜，但尚未确定任何病毒的精确脂质组成。采用质谱法，这项研究提供了一个定量分析的脂质成分的艾滋病毒和全面比较其宿主膜。大量的不寻常的鞘脂二氢鞘磷脂的富集和鞘脂生物合成在宿主细胞中的抑制病毒感染性的损失的报告，建立了一个关键的作用，这种脂质类在HIV复制周期。有趣的是，天然HIV膜的总体脂质组成类似于耐洗涤剂的膜微区，并且与宿主细胞膜的脂质组成显著不同。有了这种组合物，HIV脂质体组为活细胞中脂筏的存在提供了强有力的证据。
The lipids of enveloped viruses play critical roles in viral morphogenesis and infectivity. They are derived from the host membranes from which virus budding occurs, but the precise lipid composition has not been determined for any virus. Employing mass spectrometry, this study provides a quantitative analysis of the lipid constituents of HIV and a comprehensive comparison with its host membranes. Both a substantial enrichment of the unusual sphingolipid dihydrosphingomyelin and a loss of viral infectivity upon inhibition of sphingolipid biosynthesis in host cells are reported, establishing a critical role for this lipid class in the HIV replication cycle. Intriguingly, the overall lipid composition of native HIV membranes resembles detergent-resistant membrane microdomains and is strikingly different from that of host cell membranes. With this composition, the HIV lipidome provides strong evidence for the existence of lipid rafts in living cells.