Structural and functional aspects of the multidrug efflux pump AcrB
Structural and functional aspects of the multidrug efflux pump AcrB
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DOI：
10.1515/bc.2009.090
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发表时间：
2009-08-01
影响因子：
3.7
通讯作者：
Pos, Klaas M.
中科院分区：
文献类型：
--
作者：
Eicher, Thomas;Brandstaetter, Lorenz;Pos, Klaas M.
三联外排系统 AcrA/AcrB/TolC 是大肠杆菌中多种抗生素、染料、胆汁盐和清洁剂外排的主要泵。内膜成分 AcrB 对于底物识别和能量转导至关重要，并充当质子/药物反向转运蛋白。最近的结构研究表明，同源三聚体 AcrB 可以采用代表变构功能旋转运输循环中连续状态的不同单体构象。构象变化产生了替代的药物转运通道，包括循环中间体之一中的疏水底物结合袋。
The tripartite efflux system AcrA/AcrB/TolC is the main pump in Escherichia coli for the efflux of multiple antibiotics, dyes, bile salts and detergents. The inner membrane component AcrB is central to substrate recognition and energy transduction and acts as a proton/drug antiporter. Recent structural studies show that homotrimeric AcrB can adopt different monomer conformations representing consecutive states in an allosteric functional rotation transport cycle. The conformational changes create an alternate access drug transport tunnel including a hydrophobic substrate binding pocket in one of the cycle intermediates.