Malaria parasite-infected erythrocytes inhibit glucose utilization in uninfected red cells
Malaria parasite-infected erythrocytes inhibit glucose utilization in uninfected red cells
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DOI：
10.1016/j.febslet.2005.09.088
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发表时间：
2005-11-07
期刊：
影响因子：
3.5
通讯作者：
Sharma, S
中科院分区：
文献类型：
--
作者：
Mehta, M;Sonawat, HM;Sharma, S
疟疾寄生虫的红细胞阶段依赖于无氧糖酵解获取能量。使用[2-C-13]葡萄糖和核磁共振，测量了正常红细胞和恶性疟原虫感染的红细胞群（IRBC，寄生虫感染的红细胞<4%）中产生的葡萄糖利用率和2,3-二磷酸甘油酸（2,3-DPG）水平。 IRBC 中的葡萄糖通量高出数倍，与寄生虫血症成正比，并且在滋养体阶段达到最大。 IRBC 中 2,3-DPG 水平不成比例地较低，表明非寄生红细胞中 2,3-DPG 通量下调。这可能是由于 pH 值降低导致调节性糖酵解酶磷酸果糖激酶选择性差异抑制。 IRBC 群体中大多数（> 96%）未感染红细胞的葡萄糖利用率下调可能对疟疾患者产生生理影响。 (c) 2005 年由 Elsevier B.V. 代表欧洲生化学会联合会出版。
The erythrocytic stages of the malaria parasite depend on anaerobic glycolysis for energy. Using [2-C-13]glucose and nuclear magnetic resonance, the glucose utilization rate and 2,3-diphosphoglycerate (2,3-DPG) level produced in normal RBCs and Plasmodium falciparum infected red blood cell populations (IRBCs, with < 4% parasite infected red cells), were measured. The glucose flux in IRBCs was several-folds greater, was proportional to parasitemia, and maximal at trophozoite stage. The 2,3-DPG levels were disproportionately lower in IRBCs, indicating a downregulation of 2,3-DPG flux in non-parasitized RBCs. This may be due to lowered pH leading to selective differential inhibition of the regulatory glycolytic enzyme phospho-fructokinase. This downregulation of the glucose utilization rate in the majority (> 96%) of uninfected RBCs in an IRBC population may have physiological implications in malaria patients. (c) 2005 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.