Comparative susceptibilities of human embryonic fibroblasts and HeLa cells for isolation of human rhinoviruses
Comparative susceptibilities of human embryonic fibroblasts and HeLa cells for isolation of human rhinoviruses
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DOI：
10.1128/jcm.34.5.1277-1279.1996
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发表时间：
1996-05-01
影响因子：
9.4
通讯作者：
Hayden, FG
中科院分区：
文献类型：
--
作者：
Arruda, E;Crump, CE;Hayden, FG
比较了在不同细胞类型的人胚肺成纤维细胞（HELF）WI-38株中从自然获得性感冒志愿者的鼻洗液和鼻咽拭子中回收人鼻病毒（HRV）的情况。（敏感性，61至84%）和HeLa-I，一种HRV敏感的HeLa细胞克隆（敏感性，86至94%），是所用的最敏感的细胞类型，HELF WI-38细胞比所用的其他细胞更早地显示出细胞病变效应，并且不同的HRV敏感性HeLa细胞株在它们对HRV分离的敏感性方面不同，在20%至35%的阳性样本中，在单个细胞类型中检测到HRV，这表明使用不同的HRV敏感细胞系的组合是回收HRV的最佳方法。虽然鼻洗液倾向于产生比鼻拭子和咽拭子更多的HRV分离株，但未发现两种采样方法有显著差异。
The recovery of human rhinovirus (HRV) from nasal washings and nasal and pharyngeal swabs from volunteers with naturally acquired colds was compared in different cell types, Human embryonic lung fibroblast (HELF) strain WI-38 (sensitivity, 61 to 84%) and HeLa-I, an HRV-susceptible HeLa cell clone (sensitivity, 86 to 94%), were the most sensitive cell types used, HELF WI-38 cells showed a cytopathic effect earlier than the other cells used, and the different strains of HRV-susceptible HeLa cells varied in their sensitivities for HRV isolation, HRV was detected in a single cell type in 20 to 35% of the positive samples, suggesting that use of a combination of different HRV-susceptible cell lines is the best approach for the recovery of HRV, Although nasal washings tended to yield more HRV isolates than nasal and pharyngeal swabs, the two sampling methods were not found to be significantly different.