The effect of physiological cyclic stretch on the cell morphology, cell orientation and protein expression of endothelial cells
The effect of physiological cyclic stretch on the cell morphology, cell orientation and protein expression of endothelial cells
复制标题
DOI：
10.1007/s10856-007-3125-3
复制
发表时间：
2007-10-01
影响因子：
3.7
通讯作者：
McHugh, Peter E.
中科院分区：
文献类型：
--
作者：
Barron, Valerie;Brougham, Claire;McHugh, Peter E.
在体内，内皮细胞不断地暴露在脉动剪切和拉伸应力下。本研究的主要目的是设计和建立一个生理模拟器，再现体内经历的拉伸应变的均匀应变分布，并研究这种循环拉伸应变对内皮细胞形态、细胞取向和蛋白质表达的影响。在这个新开发的模拟器中，用免疫组织化学染色和共聚焦成像实验检测了人脐静脉内皮细胞对单轴循环拉伸的生物响应，发现细胞以58.9度+/-4.5度伸长和取向。将这个值与一个数学模型进行比较，发现内皮细胞将以60度的角度定向。该模型还显示，当10%的循环应变在1 Hz下作用3h时，内皮细胞的轴向应变阈值为1.8%。与静态细胞相比，在循环应变条件下培养的细胞显示出更多的ICAM-1免疫染色，而VCAM-1受体染色水平也显著降低。总而言之，血流动力学压力可以调节体内内皮细胞的黏附反应；这一数据证实了生物反应器是复制生理环境的。
In vivo, endothelial cells are constantly exposed to pulsatile shear and tensile stresses. The main aim of this study was to design and build a physiological simulator, which reproduced homogenous strain profiles of the tensile strain experienced in vivo, and to investigate the effect of this cyclic tensile strain on the cell morphology, cell orientation and protein expression of endothelial cells. The biological response of human umbilical vein endothelial cells to a uniaxial cyclic stretch, in this newly developed simulator, was examined experimentally using immunohistostaining and confocal imaging and it was found that the cells elongated and oriented at 58.9 degrees +/- 4.5 degrees. This value was compared to a mathematical model where it was revealed that endothelial cells would orient at an angle of 60 degrees. This model also revealed that endothelial cells have an axial strain threshold value of 1.8% when exposed to a 10% cyclic strain at 1 Hz for 3 h. Cells cultured under conditions of cyclic strain showed increased ICAM-1 immunostaining when compared to static cells whereas, a marked decrease in the levels of VCAM-1 receptor staining was also observed. Haemodynamic stresses can modulate the endothelial cell adhesion response in vivo thus, taken together; this data validates the bioreactor as replicating the physiological environment.