"Rickettsia amblyommii" and R. montanensis Infection in Dogs Following Natural Exposure to Ticks
"Rickettsia amblyommii" and R. montanensis Infection in Dogs Following Natural Exposure to Ticks
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DOI：
10.1089/vbz.2013.1325
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发表时间：
2014-01-01
影响因子：
2.1
通讯作者：
Shaw, Edward
中科院分区：
文献类型：
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作者：
Barrett, Anne;Little, Susan E.;Shaw, Edward
确定犬感染斑点热群立克次体的风险。在自然蜱暴露后，10只确定没有暴露于或感染蜱传疾病病原体的证据的狗通过每周在俄克拉荷马州中北部的森林地区散步暴露于蜱。每次散步后，对犬进行检查，并记录存在的蜱虫数量和种属。然后将犬放回室外犬舍，以使感染和随后的任何病原体传播继续进行。每周两次采集每只犬的血清样品和全血，持续121天，并通过间接荧光抗体（IFA）评价与立克次体、立克次体和立克次体反应的抗体。montanensis和R.通过PCR检测立克次体属（Rickettsia spp.）在整个8周侵染期内，犬共侵染57-108只蜱虫（每周平均蜱虫侵染=12.0 +/- 4.1）。绝大多数蜱类为美洲钝眼蜱（90.5%），少数为变异革蜱和美洲钝眼蜱。斑点也被识别了。所有（10/10）犬均发生了R. rickettsii，R. montanensis和R. Amblyommii，研究中所有犬的平均最大反向滴度分别为1176、1448和6654。相对于R的最大反向滴度。Amblyommii的范围为4096至16，384，并且在9/10只狗中高于对R的最大反向滴度。rickettsii或R.蒙大拿人。序列确认的SFG立克次体属（R. montanensis和R. Amblyommii）偶尔但不一致地通过PCR从全血中鉴定。总之，我们的数据表明，在A. americanum是常见的，对R.犬的立克次氏体感染可能是由于感染了立克次氏体。安氏立克次体或其他密切相关的SFG立克次体属。
To determine the risk of canine infection with spotted fever group (SFG) Rickettsia spp. following natural tick exposure, 10 dogs determined to be free of evidence of exposure to or infection with tick-borne disease agents were exposed to ticks via weekly walks in a wooded area in north-central Oklahoma. After each walk, dogs were examined and the number and species of ticks present were recorded. The dogs were then returned to outdoor kennels to allow the infestations and subsequent transmission of any pathogens to proceed. Serum samples and whole blood were collected from each dog twice weekly for 121 days and evaluated via indirect fluorescence antibody (IFA) for antibodies reactive to Rickettsia rickettsii, R. montanensis, and R. amblyommii, and by PCR for evidence of Rickettsia spp. Dogs became infested with a total of 57-108 ticks over the entire 8-week infestation period (weekly average tick infestation=12.0 +/- 4.1). The great majority of the ticks present were Amblyomma americanum (90.5%), with a small number of Dermacentor variabilis and A. maculatum also identified. All (10/10) dogs seroconverted to R. rickettsii, R. montanensis, and R. amblyommii, with mean maximum inverse titers of 1176, 1448, and 6654, respectively, for all dogs in the study. Maximum inverse titers to R. amblyommii ranged from 4096 to 16,384 and were higher in 9/10 dogs than maximum inverse titers to R. rickettsii or R. montanensis. Sequence-confirmed SFG Rickettsia spp. (R. montanensis and R. amblyommii) were occasionally, but not consistently, identified from whole blood by PCR. Taken together, our data suggest that, in areas where A. americanum is common, antibodies reactive to R. rickettsii in dogs may be due instead to infection with R. amblyommii or other, closely related SFG Rickettsia spp.