Application of 4-(4-nitrophenyl)-1,2,4-triazoline-3,5-(dione to analysis of 25-hydroxyvitamin D3 in human plasma by liquid chromatography/electron capture atmospheric pressure chemical ionization-mass spectrometry
Application of 4-(4-nitrophenyl)-1,2,4-triazoline-3,5-(dione to analysis of 25-hydroxyvitamin D3 in human plasma by liquid chromatography/electron capture atmospheric pressure chemical ionization-mass spectrometry
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DOI：
10.2116/analsci.19.941
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发表时间：
2003-06-01
影响因子：
1.6
通讯作者：
Shimada, K
中科院分区：
文献类型：
--
作者：
Higashi, T;Yamauchi, A;Shimada, K
研究了4-（4-硝基苯基）-1，2，4-三唑啉-3，5-二酮（NPPs）作为衍生试剂在液相色谱/电子捕获大气压化学电离质谱（LC/ECAPCI-MS）分析25-羟基维生素D-3 [25（OH）D-3]中的应用。25（OH）D-3与NP 3的衍生物在APCI源中以负离子模式进行电子捕获，并提供比完整化合物高30倍的灵敏度。将此衍生化-LC/ECAPCI-MS方法应用于血浆中25-羟基维生素D-3的测定，在灵敏度、特异性、测量范围和通量等方面均获得了满意的结果。
The utility of 4-(4-nitrophenyl)-1,2,4-triazoline-3,5-dione (NPTAD) as a derivatization reagent in the analysis of 25-hydroxyvitamin D-3 [25(OH)D-3] using liquid chromatography/electron capture atmospheric pressure chemical ionization-mass spectrometry (LC/ECAPCI-MS) was examined. The derivatives of 25(OH)D-3 with NPTAD underwent electron capture in the APCI source in the negative-ion mode and provided 30-fold higher sensitivity compared to an intact compound. This derivatization-LC/ECAPCI-MS method was applied to a plasma 25-hydroxyvitamin D-3 assay, and gave satisfactory results in sensitivity, specificity, measurable range and throughput of the analysis.