Correlations between macrophage polarizing cytokines, inflammatory mediators, osteoclast activity, and toll-like receptors in tissues around aseptically loosened hip implants
Correlations between macrophage polarizing cytokines, inflammatory mediators, osteoclast activity, and toll-like receptors in tissues around aseptically loosened hip implants
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DOI：
10.1002/jbm.a.35913
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发表时间：
2017-02-01
影响因子：
4.9
通讯作者：
Pajarinen, Jukka
中科院分区：
文献类型：
--
作者：
Jamsen, Eemeli;Kouri, Vesa-Petteri;Pajarinen, Jukka
关节置换物的无菌性松动和骨质溶解是由巨噬细胞介导的对植入物产生的磨损碎片的炎症反应驱动的，但这些事件的许多方面仍不清楚。为了更好地了解无菌性松动发病机制中炎症和趋化介质、巨噬细胞表型和极化细胞因子、破骨细胞活性和 Toll 样受体 (TLR) 之间的关系，我们使用 Pearson 相关性确定了种植体周围组织中这些因素的相对表达如何相互关联以及与种植体寿命的相关性。促炎介质和趋化因子的表达显示出它们之间以及与TLR4的正相关性。此外，M1 极化 IFN-显示出与许多促炎和趋化介质呈正相关，而 M2 极化 IL-4 则没有显示出这种关联。 IL-8 表达与早期翻修时间显着相关。 TNF-、IFN-和 CCL3 也观察到类似的趋势，而 IL-4 则检测到相反的趋势。然而，没有一种炎症介质与破骨细胞活性标志物或 RANKL/OPG 比率相关。结果强调了炎症介质 IFN- 和 TLR4 在无菌性松动发病机制中的重要性；促炎状态的增加与早期翻修时间相关，而 IL-4 与更长的种植体存活时间相关。 (c) 2016 年 Wiley periodicals, Inc. J Biomed Mater Res Part A：105A：454-463，2017 年。
Aseptic loosening and osteolysis of joint replacements are driven by macrophage-mediated inflammatory reactions to implant-derived wear debris, but many aspects of these events remain poorly characterized. To better understand the relationships among inflammatory and chemotactic mediators, macrophage phenotype and polarizing cytokines, osteoclast activity, and Toll-like receptors (TLRs) in the pathogenesis of aseptic loosening, we determined how the relative expressions of these factors in the peri-implant tissues correlate to each other and to the life span of the implants using Pearson correlation. The expression of pro-inflammatory mediators and chemokines showed positive correlations among themselves, and with TLR4. Furthermore, M1-polarizing IFN- showed positive correlations with a number of pro-inflammatory and chemotactic mediators, whereas M2-polarizing IL-4 showed no such association. IL-8 expression significantly correlated with early time to revision. Similar trends were observed for TNF-, IFN-, and CCL3, while the opposite was detected for IL-4. However, none of the inflammatory mediators correlated with the markers of osteoclast activity or the RANKL/OPG ratio. The results highlight the importance of the inflammatory mediators, IFN- and TLR4, in the pathogenesis of aseptic loosening; increased pro-inflammatory status was associated with early time to revision, whereas IL-4 correlated with longer implant survival. (c) 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 454-463, 2017.