Ion channel engineering: perspectives and strategies.
Ion channel engineering: perspectives and strategies.
复制标题
离子通道工程：观点和策略。
DOI：
10.1016/j.jmb.2014.09.001
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发表时间：
2015
影响因子：
5.6
通讯作者：
Colecraft,HenryM
中科院分区：
文献类型：
--
作者：
Subramanyam,Prakash;Colecraft,HenryM
离子通道促进离子沿着电化学梯度和穿过细胞中的脂质双层的被动移动。这种现象对于生命是必不可少的，并且是细胞中许多关键的稳态过程的基础。离子通道是多样的，并且在它们如何打开和关闭（门控）以及它们的离子电导/选择性（渗透）方面不同。对离子通道结构-功能机制的基本理解，它们的生理作用，它们的功能障碍如何导致疾病，它们作为生物传感器的效用，以及开发新的分子来调节它们的活性是重要和活跃的研究前沿。在这篇综述中，我们专注于离子通道工程的方法，已被应用于调查这些方面的离子通道功能，主要强调电压门控离子通道。
Ion channels facilitate the passive movement of ions down an electrochemical gradient and across lipid bilayers in cells. This phenomenon is essential for life and underlies many critical homeostatic processes in cells. Ion channels are diverse and differ with respect to how they open and close (gating) and to their ionic conductance/selectivity (permeation). Fundamental understanding of ion channel structure–function mechanisms, their physiological roles, how their dysfunction leads to disease, their utility as biosensors, and development of novel molecules to modulate their activity are important and active research frontiers. In this review, we focus on ion channel engineering approaches that have been applied to investigate these aspects of ion channel function, with a major emphasis on voltage-gated ion channels.