Characterization of the FMN-Dependent Cysteine Decarboxylase from Thioviridamide Biosynthesis
Characterization of the FMN-Dependent Cysteine Decarboxylase from Thioviridamide Biosynthesis
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硫代病毒酰胺生物合成中 FMN 依赖性半胱氨酸脱羧酶的表征
DOI：
10.1021/acs.orglett.9b01531
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发表时间：
2019
期刊：
影响因子：
5.2
通讯作者：
Huan Wang
中科院分区：
文献类型：
--
作者：
Jingxia Lu;Jiao Li;Yuan Wu;Xianyang Fang;Jiapeng Zhu;Huan Wang
The biosynthesis of thioviridamide-like compounds has not been elucidated. Herein, we report that TvaF from the thioviridamide biosynthetic gene cluster is an FMN-dependent cysteine decarboxylase that transforms the C-terminal cysteine of precursor peptides into a thioenol motif and exhibits high substrate flexibility. We resolved the crystal structure of TvaF bound with FMN at 2.24 Å resolution. Key residues for FMN binding and catalytic activity of TvaF have been identified and evaluated by mutagenesis studies.
