Estrogen receptor beta does not influence ischemic tolerance in the aged female rat heart.
Estrogen receptor beta does not influence ischemic tolerance in the aged female rat heart.
复制标题
雌激素受体β不影响老年雌性大鼠心脏的缺血耐受性。
DOI：
10.1111/j.1755-5922.2011.00288.x
复制
发表时间：
2013
影响因子：
3.1
通讯作者：
Korzick,DonnaH
中科院分区：
文献类型：
--
作者：
Tomicek,NanetteJ;Miller-Lee,JenniferL;Hunter,JCraig;Korzick,DonnaH
简介：缺血性心脏病仍然是老年女性发病和死亡的主要原因，绝经后发病率增加 2 至 3 倍。临床试验未能证明长期雌激素 (E2) 替代疗法对心脏的保护作用，但 E2 的保护作用已在成年动物模型中得到证实，并且是由雌激素受体 (ER) 亚型 ERα 和 ERβ 介导的。目的：本研究的目的是确定急性 ERβ 激活对成年、老年和老年 E2 缺陷雌性大鼠的缺血/再灌注 (I/R) 损伤的影响。方法：心脏分离自成年（6 个月；n = 9）、老年（24 个月；n = 13）和老年卵巢切除（OVX；n = 14）雌性 Fischer 344 大鼠，并接受 47 分钟的全局 I 和 60 分钟的 R。在 I/R 前 45 分钟，用 ERβ 激动剂二芳基丙腈（DPN；5μg/kg）或媒介物对大鼠进行急性治疗；还评估了 ERβ mRNA 和蛋白水平。结果：DPN 急性治疗对成年、老年或老年 OVX 雌性大鼠 I/R 损伤后的功能恢复没有影响。此外，我们无法在成年或老年雌性大鼠心肌中检测到 ERβ mRNA 或蛋白。结论：在这里，我们的数据首次表明，急性 ERβ 激活不会影响成年或老年雌性 Fischer 344 大鼠心肌的缺血耐受性，这可能是由于缺乏可检测到的 ERβ。
Introduction:Ischemic heart disease remains the leading cause of morbidity and mortality in aged women, with a 2‐ to 3‐fold increase in incidence following menopause. Clinical trials have failed to demonstrate cardioprotective benefit from chronic estrogen (E2) replacement therapy, yet protective effects of E2have been demonstrated in adult animal models and are mediated by the estrogen receptor (ER) subtypes ERα and ERβ.Aims:The aim of this study was to determine the effects of acute ERβ activation on ischemia/reperfusion (I/R) injury in adult, aged, and aged E2‐deficient female rats.Methods:Hearts were isolated from adult (6 months; n = 9), aged (24 months; n = 13), and aged ovariectomized (OVX; n = 14) female Fischer 344 rats and subjected to 47 min of global I and 60 min of R. Rats were acutely treated with the ERβ‐agonist diarylpropionitrile (DPN; 5μg/kg) or vehicle 45 min prior to I/R; ERβ mRNA and protein levels were also assessed.Results:Acute treatment with DPN had no effect on functional recovery following I/R injury in adult, aged, or aged OVX female rats. Additionally, we were unable to detect ERβ mRNA or protein in the adult or aged female rat myocardium.Conclusions:Here, for the first time, our data suggest that acute ERβ activation does not impact ischemic tolerance in the adult or aged female Fischer 344 rat myocardium and this likely due to a lack of detectable ERβ.