Comparison of disc diffusion and agar dilution methods for antibiotic susceptibility testing of Campylobacter jejuni subsp. jejuni and Campylobacter coli
Comparison of disc diffusion and agar dilution methods for antibiotic susceptibility testing of Campylobacter jejuni subsp. jejuni and Campylobacter coli
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DOI：
10.1093/jac/39.6.707
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发表时间：
1997-06-01
影响因子：
5.2
通讯作者：
Gilbert, H
中科院分区：
文献类型：
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作者：
Gaudreau, C;Gilbert, H
用纸片扩散法和琼脂稀释法对145株空肠弯曲菌、空肠弯曲菌和46株结肠弯曲菌进行纸片扩散和琼脂稀释药敏试验。耐药株纸片扩散的真实性和95%可信区间分别为：四环素(53株)100%(93.2-100%)、环丙沙星(13株)100%(77.2-100%)、奈立克酸(15株)86.7%(62.1-96.3%)、红霉素(5株)100%(56.6-100%)、氨苄西林(16株)68.8%(44.4-85.8%)。89-140株对5种抗生素敏感株的真实结果和95%可信区间分别为97.6-100%和93.2-100%。奈立地酸的大误差为1.4%，红霉素的极大误差为0.7%，氨苄西林的小误差和大误差分别为5.5%和1.4%。环丙沙星和四环素完全一致。氨苄西林的药敏结果预计不会在临床上有用。耐环丙沙星敏感株对环丙沙星敏感，而耐药株大多对环丙沙星耐药，耐甲氧西林纸片是环丙沙星敏感性的标志。总之，我们的结果表明纸片扩散法是一种对红霉素、环丙沙星和四环素进行药敏试验的可靠、简便和廉价的方法。在对更多的红霉素和环丙沙星耐药菌株进行测试以确认这项测试的可靠性之前，需要使用Etest或琼脂稀释法来确认对这些药物的耐药性。
The correlation between disc diffusion and agar dilution susceptibility testing of five antibiotics was studied against 145 Campylobacter strains: 99 Campylobacter jejuni subsp, jejuni and 46 Campylobacter coli. The percentages of true results and 95% CI for disc diffusion for resistant strains were 100% (93.2-100%) for tetracycline (53 strains tested), 100% (77.2-100%) for ciprofloxacin (13 strains tested), 86.7% (62.1-96.3%) for nalidixic acid (15 strains tested), 100% (56.6-100%) for erythromycin (five strains tested) and 68.8% (44.4-85.8%) for ampicillin (16 strains tested). The percentages of true results and 95% CI were 97.6-100% and 93.2-100% respectively for 89-140 susceptible strains to the five antibiotics tested. There was a 1.4% major error for nalidixic acid, 0.7% very major error for erythromycin, 5.5% and 1.4% minor and major errors respectively for ampicillin. There was complete agreement for ciprofloxacin and tetracycline. Results of ampicillin susceptibility are not expected to be useful in a clinical setting. The nalidixic acid disc is a marker of ciprofloxacin susceptibility as the nalidixic acid-susceptible strains were susceptible to ciprofloxacin while most of the resistant ones were resistant to ciprofloxacin. Overall, our results suggest that disc diffusion is a reliable, easy and inexpensive susceptibility testing method for C. jejuni and C. coli for erythromycin, ciprofloxacin and tetracycline. Until more erythromycin- and ciprofloxacin-resistant strains are tested to confirm the reliability of this test, the resistance to these drugs needs to be confirmed using the Etest or the agar dilution method.