Partitioning of P1 plasmids by gradual distribution of the ATPase ParA
Partitioning of P1 plasmids by gradual distribution of the ATPase ParA
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DOI：
10.1111/j.1365-2958.2010.07398.x
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发表时间：
2010-12-01
影响因子：
3.6
通讯作者：
Niki, Hironori
中科院分区：
文献类型：
--
作者：
Hatano, Toshiyuki;Niki, Hironori
P>；最近，有报道称原核生物也有一个类似有丝分裂的器官，其中聚合的纤维控制着染色体和质粒的双极运动。在这里，我们展示了不形成聚合细丝的非有丝分裂样器进行质粒分割的证据。P1para是一种与DNA结合的ATPase蛋白，分布于整个类核中，并在类核中心形成一个致密的斑点。对位斑点的荧光强度闪烁，然后斑点逐渐从中细胞迁移到细胞四分之一位置。在无核细胞中没有观察到这样的分布，这表明类核可能是逐步分布的基质。质粒DNA不断地在PARA处共定位，并根据斑点迁移和分离进行迁移。因此，PARA的梯度分布决定了分配质粒的目的地，并可能将质粒定向到细胞区。
P>Recently, it has been reported that prokaryotes also have a mitotic-like apparatus in which polymerized fibres govern the bipolar movement of chromosomes and plasmids. Here, we show evidence that a non-mitotic-like apparatus that does not form polymerized filaments carries out plasmid partitioning. P1 ParA, which is a DNA-binding ATPase protein, was found to be distributed through the whole nucleoid and formed a dense spot at the centre of the nucleoid. The fluorescent intensity of the ParA spot blinked, and then the spot gradually migrated from the midcell to a cell quarter position. Such distribution was not observed in anucleate cells, suggesting that the nucleoid could be a matrix for gradual distribution of ParA. Plasmid DNA constantly colocalized at the spot of ParA and migrated according to spot migration and separation. Thus, the gradient distribution of ParA determines the destination of partitioning plasmids and may direct plasmids to the cell quarters.