COMPARATIVE-STUDY OF GLYCOLYSIS IN RED AND WHITE MUSCLES OF TROUT (SALMO-GAIRDNERI) AND MIRROR CARP (CYPRINUS-CARPIO)
COMPARATIVE-STUDY OF GLYCOLYSIS IN RED AND WHITE MUSCLES OF TROUT (SALMO-GAIRDNERI) AND MIRROR CARP (CYPRINUS-CARPIO)
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DOI：
10.1111/j.1095-8649.1977.tb05714.x
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发表时间：
1977-01-01
影响因子：
2
通讯作者：
JOHNSTON, IA
中科院分区：
文献类型：
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作者：
JOHNSTON, IA
一些糖酵解和相关酶的活动，确定在鳟鱼和鲤鱼的肌肉中，以调查的可能性，以前报道的乳酸积累和缺氧耐受性之间的差异，在这2条鱼导致糖酵解潜力的潜在差异。某些糖酵解中间体的稳态浓度也确定在冷冻夹紧的肌肉从坦克休息的鱼。鲤鱼肌肉中己糖激酶、磷酸化酶和磷酸果糖激酶的活性比鳟鱼白色肌肉低2-3倍。鲤鱼白色肌肉中丙酮酸激酶和乳酸脱氢酶活性低5倍。较低，更广泛的pH最适的乳酸脱氢酶和丙酮酸激酶从鲤鱼相比，鳟鱼肌肉与更大的缺氧耐受性的鲤鱼。糖酵解酶谱显着不同的红色和白色肌肉的虹鳟鱼，但大致相似，与己糖激酶活性的例外，为相应的肌肉的鲤鱼。本文还讨论了这两种鱼的厌氧性和红、白色肌的比较生理。
The activities of some glycolytic and associated enzymes were determined in the muscles of trout and carp to investigate the possibility that the discrepancies previously reported between lactate accumulation and anoxic tolerance in these 2 fish resulted from underlying differences in glycolytic potential. Steady state ccentrations of certain glycolytic intermediates were also determined in freeze-clamped muscles from tank-rested fish. The activities of hexokinase, phosphorylase and phosphofructokinase were approximately 2-3 times lower in carp than trout white muscles. Pyruvate kinase and lactate dehydrogenase activities were 5 times lower in carp white muscle. The lower, broader pH optima of lactate dehydrogenase and pyruvate kinase from carp compared to trout muscles was correlated with the greater anoxic tolerance of the carp. Glycolytic enzyme profiles were markedly different between the red and white muscles of the rainbow trout but broadly similar, with the exception of hexokinase activity, for the corresponding muscles of the carp. The results were discussed in relation to anaerobiosis in these 2 species and the comparative physiology of red and white muscles in fish.