Rabbit ovarian protein kinases. II. Effect of an ovulatory dose of human chorionic gonadotropin or luteinizing hormone on the multiplicity of follicular and luteal protein kinases.
Rabbit ovarian protein kinases. II. Effect of an ovulatory dose of human chorionic gonadotropin or luteinizing hormone on the multiplicity of follicular and luteal protein kinases.
复制标题
兔卵巢蛋白激酶。
DOI：
10.1210/endo-103-2-431
复制
发表时间：
1978
期刊：
影响因子：
4.8
通讯作者：
R. Jungmann
中科院分区：
文献类型：
--
作者：
M. Hunzicker;R. Jungmann
DEAE-纤维素层析显示，发情兔卵泡中存在一种单一形式的cAMP依赖的蛋白激酶，命名为蛋白激酶3。静脉注射一定剂量的hCG可促进发情兔出现第二个瞬时峰值，即蛋白激酶1。蛋白激酶1在注射hCG后10分钟内被检测到，但在假孕的黄体(CL)和妊娠的黄体(CL)中分别在24小时和72小时回落到检测不到的水平。排卵和随后的CL形成伴随着第三种形式的cAMP依赖的蛋白激酶的出现，命名为蛋白激酶2。蛋白激酶2在hCG注射后2小时内出现，并作为胞浆cAMP依赖的蛋白激酶的主要形式存在于CL的整个生命周期。这三种形式的蛋白激酶均可被兔骨骼肌热稳定蛋白激酶抑制剂抑制，具有cAMP结合活性，并可被10(-7)M cAMP显著刺激。妊娠黄体、白体和间质组织中蛋白激酶3的活性显著高于卵泡和假孕黄体，而蛋白激酶2的活性在整个黄体周期内相对恒定。给假孕4天兔静脉注射一定剂量的hCG，对注射hCG后10分钟至3天的黄体胞浆DEAE-纤维素层析法测定的蛋白激酶放散图谱无明显影响。然而，随着白化体脱分化为间质组织，蛋白激酶2对cAMP的依赖性降低。结果表明，卵巢结构胞浆中cAMP依赖的蛋白激酶的活性和多样性受黄体生成素(HCG)的调节，并与兔不同的生殖阶段有关。
DEAE-cellulose chromatography of the 105,000 X g supernatant fraction (cytosol) obtained from popped estrous rabbit follicles revealed the presence of a single form of cAMP-dependent protein kinase, designated protein kinase 3. The iv injection of an ovulatory dose of hCG to estrous rabbits promoted the appearance of a second, transient peak of cytosol cAMP-dependent protein kinase, protein kinase 1. Protein kinase 1 was detected within 10 min of hCG administration but had regressed to undetectable levels by 24 h in corpora lutea (CL) of pseudopregnancy and by 72 h in CL of pregnancy. Ovulation and subsequent CL formation were accompanied by the appearance of a third form of cAMP-dependent protein kinase, designated protein kinase 2. Protein kinase 2 was present within 2 h after hCG administration and persisted as a major form of cytosol cAMP-dependent protein kinase throughout the life span of CL. All three forms of protein kinase were inhibited by the heat-stable protein kinase inhibitor from rabbit skeletal muscle, possessed cAMP-binding activity, and were markedly stimulated by 10(-7) M cAMP. The activity of protein kinase 3 in CL of pregnancy, in corpora albicantia, and in interstitial tissue was markedly greater than that in follicles or in CL of pseudopregnancy, while the activity of protein kinase 2 remained relatively constant throughout the luteal life span. The iv injection of a luteolytic dose of hCG to 4-day pseudopregnant rabbits promoted no alterations of the protein kinase elution profile upon DEAE-cellulose chromatography of the luteal cytosol obtained 10 min to 3 days post-hCG injection. However, with dedifferentiation of corpora albicantia into interstitial tissue, the cAMP dependency of protein kinase 2 was reduced. The results indicate that the enzymatic activity and multiplicity of cAMP-dependent protein kinases in the cytosol of ovarian structures are subject to regulation by LH (hCG) and depend upon the various reproductive stages of the rabbit.