OmpA-like protein influences cell shape and adhesive activity of Tannerella forsythia
OmpA-like protein influences cell shape and adhesive activity of Tannerella forsythia
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DOI：
10.1111/j.2041-1014.2011.00625.x
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发表时间：
2011-12-01
影响因子：
3.7
通讯作者：
Yoshimura, F.
中科院分区：
文献类型：
--
作者：
Abe, T.;Murakami, Y.;Yoshimura, F.
连翘坦纳氏菌是一种革兰氏阴性梭形杆菌，与多种口腔厌氧菌感染有关。大多数革兰氏阴性细菌都有类似OmpA的蛋白，这种蛋白与大肠杆菌中的OmpA蛋白同源。通过质谱分析，我们确定了tf1331基因编码的一个类似OmpA的蛋白是连翘中的主要蛋白之一。双向对角电泳法表明，OmpA样蛋白通过分子间二硫键形成二聚体或三聚体结构。生物素标记实验表明，尽管连翘在最外层的细胞表面有一层S层，但部分蛋白质暴露在细胞表面。使用tf1331缺失突变体，我们发现OmpA样蛋白影响细胞形态。突变细胞的长度减少了近一半。在40%以上的突变细胞中观察到细胞肿胀。此外，突变体表现出与纤维连接蛋白的粘附性降低，自聚集延迟，细胞表面疏水性降低。这些结果表明，连翘中的OmpA样蛋白在细胞完整性和黏附功能中起着重要的作用。
Tannerella forsythia, a gram-negative fusiform rod, is implicated in several types of oral anaerobic infections. Most gram-negative bacteria have OmpA-like proteins that are homologous to the OmpA protein in Escherichia coli. We identified an OmpA-like protein in T. forsythia encoded by the tf1331 gene as one of the major proteins by mass spectrometric analysis. Two-dimensional, diagonal electrophoresis showed that the OmpA-like protein formed a dimeric or trimeric structure via intermolecular disulfide bonds. A biotin labeling experiment revealed that a portion of the protein was exposed on the cell surface, even though T. forsythia possesses an S-layer at the outermost cell surface. Using a tf1331-deletion mutant, we showed that the OmpA-like protein affected cell morphology. The length of the mutant cell was reduced almost by half. Cell swelling was observed in more than 40% of the mutant cells. Moreover, the mutant exhibited decreased adhesion to fibronectin, retarded autoaggregation, and reduced cell surface hydrophobicity. These results suggest that the OmpA-like protein in T. forsythia plays an important role in cellular integrity and adhesive function.