Visual Stimulation Regulates the Expression of Transcription Factors and Modulates the Composition of AP-1 in Visual Cortexa
Visual Stimulation Regulates the Expression of Transcription Factors and Modulates the Composition of AP-1 in Visual Cortexa
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DOI：
10.1523/jneurosci.16-12-03968.1996
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发表时间：
1996-06
期刊：
影响因子：
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通讯作者：
B. Kamińska;L. Kaczmarek;A. Chaudhuri
中科院分区：
文献类型：
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作者：
B. Kamińska;L. Kaczmarek;A. Chaudhuri
人们认为，神经元功能的长期变化是由转录因子（如AP-1和ZIF 268）精心策划的，而转录因子又受到突触刺激的调节。为了进一步了解这种表达的功能作用，我们通过电泳迁移位移法（EMSA）检测了AP-1和ZIF 268的dna结合活性，这些提取自大鼠视觉皮质的核提取物经选择性光照处理后。黑暗饲养后的视觉刺激使AP-1和ZIF 268的dna结合活性在2小时内达到最高水平。此后，ZIF 268下降到与幼稚动物相似的水平，而AP-1 dna结合活性在刺激24小时后继续保持升高。AP-1复合体的成分，当通过emsa超移分析评估时，在不同的暴露条件下显示出相当大的变化。FosB和JunD是幼年和暗养动物AP-1的主要成分。短暂的视觉刺激（2小时）使c-Fos、c-Jun和JunB加入到这个复合体中，而长时间的刺激（6 - 24小时）显著降低了c-Fos和c-Jun的水平，只留下FosB、JunB和JunD作为AP-1的主要成分。这些结果表明，AP-1的转录控制可能是由Fos和Jun家族不同成员的选择性组合相互作用产生的，并受活性依赖过程的指导。
It is believed that long-term changes in neuronal function are orchestrated by transcription factors, such as AP-1 and ZIF 268, which are in turn regulated by synaptic stimulation. To further our understanding of the functional effects of such expression, we have examined the DNA-binding activities of both AP-1 and ZIF 268 by way of electrophoretic mobility shift assays (EMSA) on nuclear extracts from visual cortices of rats treated with selective light exposure. Visual stimulation after dark rearing increased the DNA-binding activities of both AP-1 and ZIF 268 to their highest levels within 2 hr. ZIF 268 thereafter dropped to levels similar to that observed in naive animals, whereas AP-1 DNA-binding activity continued to remain elevated even after 24 hr of stimulation. The components of the AP-1 complex, when assessed by EMSA-supershift analysis, showed considerable variability under different conditions of exposure. FosB and JunD were the major constituents of AP-1 in both naive and dark-reared animals. Brief visual stimulation (2 hr) added c-Fos, c-Jun, and JunB to this complex, whereas prolonged stimulation (6−24 hr) reduced c-Fos and c-Jun levels significantly, leaving only FosB, JunB, and JunD as the major components of AP-1. These results suggest that transcriptional control by AP-1 may be generated by selective combinatorial interactions of different members of the Fos and Jun families and that are guided by activity-dependent processes.