Physiological and anatomical aspects of the reproduction of mice with reduced Syndecan-1 expression
Physiological and anatomical aspects of the reproduction of mice with reduced Syndecan-1 expression
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DOI：
10.1186/s12958-019-0470-2
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发表时间：
2019-03-02
影响因子：
4.4
通讯作者：
Baston-Buest, Dunja M.
中科院分区：
文献类型：
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作者：
Gougoula, Christina;Bielfeld, Alexandra P.;Baston-Buest, Dunja M.
Syndecan-1是一种硫酸乙酰肝素蛋白多糖，作为细胞因子和生长因子的辅助受体，介导发育、免疫和血管生成过程。在人类中，Syndecan-1的子宫胎盘定位及其在妊娠相关病理（如子宫内生长受限）中的表达减少表明Syndecan-1在胚胎-母体相互作用中的影响。本研究的目的是确定减少Syndecan-1的表达对小鼠及其后代的生殖表型的影响。MethodsReproductive characteristics已经使用减少Syndecan-1和它们的野生型对照动物正常交配后，反之亦然胚胎移植后进行了研究。雌性小鼠用于测量发情周期长度和妊娠期间的体重增加，以及用于卵巢的组织学检查。检查雄性小鼠精子的浓度、活力、存活率和形态。分离6月龄动物的心、肺、肝、肾、脾、脑和卵巢或睾丸和附睾等器官并称重。采用双尾学生t检验进行统计学分析，P
BackgroundSyndecan-1 is a heparan sulfate proteoglycan acting as a co-receptor for cytokines and growth factors mediating developmental, immunological and angiogenic processes. In human, the uteroplacental localization of Syndecan-1 and its reduced expression in pregnancy-associated pathologies, such as the intrauterine growth restriction, suggests an influence of Syndecan-1 in embryo-maternal interactions. The aim of the present study was to identify the effect of a reduced expression of Syndecan-1 on the reproductive phenotype of mice and their progenies.MethodsReproductive characteristics have been investigated using animals with reduced Syndecan-1 and their wildtype controls after normal mating and after vice versa embryo transfers. Female mice were used to measure the estrus cycle length and the weight gain during pregnancy, as well as for histological examination of ovaries. Male mice were examined for the concentration, motility, viability and morphology of spermatozoa. Organs like heart, lung, liver, kidney, spleen, brain and ovaries or testes and epididymis of 6-month-old animals were isolated and weighed. Statistical analyses were performed using two-tailed students t-test with P