The SUPPRESSOR of MAX2 1 (SMAX1)-Like SMXL6, SMXL7 and SMXL8 Act as Negative Regulators in Response to Drought Stress in Arabidopsis
The SUPPRESSOR of MAX2 1 (SMAX1)-Like SMXL6, SMXL7 and SMXL8 Act as Negative Regulators in Response to Drought Stress in Arabidopsis
复制标题
MAX2 1 (SMAX1) 的抑制因子 - 与 SMXL6、SMXL7 和 SMXL8 一样，在拟南芥中充当响应干旱胁迫的负调节因子。
DOI：
10.1093/pcp/pcaa066
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发表时间：
2020
影响因子：
4.9
通讯作者：
Wang Chongying
中科院分区：
文献类型：
--
作者：
Yang Tao;Lian Yuke;Kang Jihong;Bian Zhiyuan;Xuan Lijuan;Gao Zhensheng;Wang Xinyu;Deng Jianming;Wang Chongying
干旱是对作物生长和产量的主要威胁。独脚金内酯（SL）通过靶向促进更多轴突生长的供应子2（MAX2）-LIKE 6（SMXL 6）、SMXL 7和SMXL 8以MAX2依赖的方式降解而有助于调节拟南芥中的芽分枝。虽然SL参与植物干旱响应，但SMXL 6、7和8在SL调节的植物干旱胁迫响应中的功能仍不清楚。在此，我们对smx 16、7、8和max2植物进行了转录组学、生理和生化分析，以了解SMXL 6/7/8调节干旱反应的基础。我们发现三个D53（DWARF 53）-Like SMXL成员SMXL 6、7和8参与干旱响应，因为与野生型（WT）相比，这些mxl 6 smxl 7 smxl 8三重突变体显示出显著增强的耐旱性。在脱水过程中，mx 16 smx 17 smx 18植株的气孔指数、角质层透性和失水量降低，花青素合成增加。此外，smxl 6 smxl 7 smxl 8在萌发过程中和萌发后对ABA诱导的气孔关闭和阿坝反应都表现出高度敏感性。此外，在正常和脱水条件下对D53-likesmxl突变体、SL-responsemax 2突变体和WT植物的叶片的RNA测序分析揭示了SMXL 6/7/8介导的网络通过许多胁迫和/或ABA-响应和SL-相关基因控制植物对干旱胁迫的适应。这些数据进一步提供了证据阿坝和SL依赖的信号通路之间的串扰调节植物对干旱的反应。我们的研究结果表明，SMXL 6，7和8是SL信号的重要组成部分，并负参与干旱反应，这表明SMXL 6/7/8依赖的SL信号的遗传操作可能提供新的方法来提高抗旱性。
Drought represents a major threat to crop growth and yields. Strigolactones (SLs) contribute to regulating shoot branching by targeting the SUPPRESSOR OF MORE AXILLARY GROWTH2 (MAX2)-LIKE6 (SMXL6), SMXL7 and SMXL8 for degradation in a MAX2-dependent manner in Arabidopsis. Although SLs are implicated in plant drought response, the functions of the SMXL6, 7 and 8 in the SL-regulated plant response to drought stress have remained unclear. Here, we performed transcriptomic, physiological and biochemical analyses ofsmxl6,7,8andmax2plants to understand the basis for SMXL6/7/8-regulated drought response. We found that three D53 (DWARF53)-Like SMXL members, SMXL6, 7 and 8, are involved in drought response as thesmxl6smxl7smxl8triple mutants showed markedly enhanced drought tolerance compared to wild type (WT). Thesmxl6smxl7smxl8plants exhibited decreased leaf stomatal index, cuticular permeability and water loss, and increased anthocyanin biosynthesis during dehydration. Moreover,smxl6smxl7smxl8were hypersensitive to ABA-induced stomatal closure and ABA responsiveness during and after germination. In addition, RNA-sequencing analysis of the leaves of the D53-likesmxlmutants, SL-responsemax2mutant and WT plants under normal and dehydration conditions revealed an SMXL6/7/8-mediated network controlling plant adaptation to drought stress via many stress- and/or ABA-responsive and SL-related genes. These data further provide evidence for crosstalk between ABA- and SL-dependent signaling pathways in regulating plant responses to drought. Our results demonstrate that SMXL6, 7 and 8 are vital components of SL signaling and are negatively involved in drought responses, suggesting that genetic manipulation of SMXL6/7/8-dependent SL signaling may provide novel ways to improve drought resistance.