Nestin expression in embryonic human neuroepithelium and in human neuroepithelial tumor cells.
Nestin expression in embryonic human neuroepithelium and in human neuroepithelial tumor cells.
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发表时间：
1992-03
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通讯作者：
T. Tohyama;V. Lee;L. Rorke;M. Marvin;R. McKay;J. Trojanowski
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作者：
T. Tohyama;V. Lee;L. Rorke;M. Marvin;R. McKay;J. Trojanowski
巢蛋白是一个最近被描述的中间丝（IF）蛋白家族的成员，特别是丰富的神经上皮干细胞的大鼠。本文描述的研究检查了这种VI类IF蛋白在正常人发育中的中枢神经系统（CNS）、人脑肿瘤衍生的细胞系和人CNS肿瘤的组织样品中。人巢蛋白具有与大鼠巢蛋白相似的生物化学和免疫化学特性。此外，如在大鼠中，巢蛋白在几种不同类型的未成熟的人CNS细胞中被免疫化学检测到，即生发基质细胞、衬在中央管的神经上皮细胞、放射状神经胶质细胞和血管细胞。巢蛋白在最早的胎龄出现在这些细胞中（即，6周），然后在胚胎后期，除了血管细胞外，所有细胞的表达都下降。免疫细胞化学检测7个原始神经外胚层肿瘤细胞系中有6个细胞系和2个恶性胶质瘤细胞系中均有巢蛋白表达。在这些细胞系中，巢蛋白与含有其他IF蛋白的IF束不完全共定位（即，波形蛋白、胶质丝、神经丝）。巢蛋白在多种脑肿瘤中普遍存在，但在胶质瘤中最为突出。巢蛋白在人类胚胎发生早期在原始神经上皮细胞中的瞬时表达及其在神经上皮肿瘤中的丰度表明，巢蛋白IF在胚胎中枢神经系统干细胞退出细胞周期之前的细胞事件中发挥作用，并使这些干细胞的后代定型为特定谱系。随后诱导不同成员的IF蛋白家族在表型不同的中枢神经系统细胞（即神经元，神经胶质细胞）和消除巢蛋白从几乎所有分化的中枢神经系统细胞，意味着不同类别的IF subserve功能，是密切相关的成熟状态，以及谱系，中枢神经系统细胞。
Nestin is a recently described member of the intermediate filament (IF) protein family that is especially abundant in neuroepithelial stem cells of the rat. The studies described here examine this class VI IF protein in the normal human developing central nervous system (CNS), human brain tumor-derived cell lines, and tissue samples of human CNS tumors. Human nestin exhibited biochemical and immunochemical properties similar to those of rat nestin. Further, as in the rat, nestin was detected immunohistochemically in several different types of immature human CNS cells, i.e. germinal matrix cells, neuroepithelial cells lining the central canal, radial glia and vascular cells. Nestin appeared in these cells at the earliest gestational age (i.e., 6 weeks) examined here and then it declined in all but the vascular cells at later embryonic stages. Nestin also was detected by immunocytochemistry in 6 of 7 primitive neuroectodermal tumor cell lines and in both of 2 malignant glioma cell lines examined. In these cell lines, nestin co-localized incompletely with bundles of IFs containing other IF proteins (i.e., vimentin, glial filament, neurofilament). Nestin was ubiquitous in a wide variety of brain tumors, but was most prominent in gliomas. The transient expression of nestin in primitive neuroepithelial cells at early stages of human embryogenesis and its abundance in neuroepithelial tumors suggest a role for nestin IFs in cellular events that precede the exit of embryonic CNS stem cells from the cell cycle and the commitment of the progeny of these stem cells to a specific lineage. The subsequent induction of different members of the IF protein family in phenotypically distinct CNS cells (i.e. neurons, glia) and the elimination of nestin from almost all differentiated CNS cells, imply that different classes of IFs subserve functions that are closely linked to the maturational state, as well as the lineage, of CNS cells.