Exercise training improves the defective centrally mediated erectile responses in rats with type I diabetes.
Exercise training improves the defective centrally mediated erectile responses in rats with type I diabetes.
复制标题
运动训练可改善 I 型糖尿病大鼠中枢介导的勃起反应缺陷。
DOI：
10.1111/j.1743-6109.2011.02442.x
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Patel,KaushikP
中科院分区：
文献类型：
--
作者：
Zheng,Hong;Mayhan,WilliamG;Patel,KaushikP
IntroductionErectile dysfunction is a serious and common complication of diabetes mellitus. Apart from the peripheral actions, central mechanisms are also responsible for the penile erection.AimThe goal of the present study was to determine the impact of exercise training (ExT) on the centrally mediated erectile dysfunction in streptozotocin (STZ)‐induced type I diabetic (T1D) rats.MethodsMale Sprague–Dawley rats were injected with STZ to induce diabetes mellitus. Three weeks after STZ or vehicle injections, rats were assigned to either ExT (treadmill running for 3–4 weeks) or sedentary groups to produce four experimental groups: control + sedentary, T1D + sedentary, control + ExT, and T1D + ExT.Main Outcome MeasureAfter 3–4 weeks ExT, central N‐methyl‐D‐aspartic acid (NMDA) or sodium nitroprusside (SNP)‐induced penile erectile responses were measured. Neuronal nitric oxide synthase (nNOS) expression in the paraventricular nucleus (PVN) of the hypothalamus was measured by using histochemistry, real time polymerase chain reaction (PCR) and Western blot approaches.ResultsIn rats with T1D, ExT significantly improved the blunted erectile response, and the intracavernous pressure changes to NMDA (50 ng) microinjection within the PVN (T1D + ExT: 3.0 ± 0.6 penile erection/rat; T1D + sedentary: 0.5 ± 0.3 penile erection/rat within 20 minutes,P< 0.05). ExT improved erectile dysfunction induced by central administration of exogenous nitric oxide (NO) donor, SNP in T1D rats. Other behavior responses including yawning and stretching, induced by central NMDA and SNP microinjection were also significantly increased in T1D rats after ExT. Furthermore, we found that ExT restored the nNOS mRNA and protein expression in the PVN in T1D rats.ConclusionThese results suggest that ExT may have beneficial effects on the erectile dysfunction in diabetes through improvement of NO bioavailability within the PVN. Thus, ExT may be used as therapeutic modality to up‐regulate nNOS within the PVN and improve the central component of the erectile dysfunction in diabetes mellitus.
