Application of amphiphilic fluorophore-derived nanoparticles to provide contrast to human embryonic stem cells without affecting their pluripotency and to monitor their differentiation into neuron-like cells
Application of amphiphilic fluorophore-derived nanoparticles to provide contrast to human embryonic stem cells without affecting their pluripotency and to monitor their differentiation into neuron-like cells
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应用两亲性荧光团衍生纳米颗粒为人类胚胎干细胞提供对比而不影响其多能性并监测其向神经元样细胞的分化
DOI：
10.1016/j.actbio.2018.07.051
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发表时间：
2018
影响因子：
9.7
通讯作者：
Li Yang
中科院分区：
文献类型：
--
作者：
Zhou Shixin;Zhao Hongxi;Feng Ruopeng;Ding Lan;Li Zhiqiang;Deng Changwen;He Qihua;Liu Yinan;Song Bo;Li Yang
荧光标记是一种潜在的生物学技术，可以直接检测和跟踪经历各种生物过程的细胞。与传统的基因修饰方法相比，用纳米颗粒标记细胞具有优势，特别是它们通过避免基因组整合而提供额外的安全性。然而，确定纳米颗粒是否会干扰细胞特性并在活细胞中提供持久的信号仍然是一个挑战。我们采用了一种两亲性荧光团衍生的纳米颗粒（用TPE-11表示），它具有四苯乙烯（TPE）部分和两个离子头；该纳米颗粒具有聚集诱导发射（AIE）效应和自组装能力。在相同条件下，TPE-11在细胞成像中表现出比其他两种纳米材料更高或更长的荧光强度。我们使用这种纳米材料来标记人类胚胎干细胞（hES）并监测其分化。用低浓度的TPE-11（8.0 μg/mL）处理后，he细胞得到了高强度的标记，免疫染色分析和畸胎瘤形成实验表明，在该浓度下，其多能性保持不变。TPE-11纳米颗粒允许长期监测hES细胞向神经元样细胞的分化；值得注意的是，在近40天的分化过程中，检测到强烈的纳米颗粒信号。因此，这些结果表明，TPE-11纳米颗粒对hES细胞具有良好的生物相容性，是一种潜在的用于标记和跟踪人类多能干细胞分化的氟剂。本研究使用基于纳米粒子的方法来标记人类胚胎干细胞（hES）并监测其分化。hES细胞具有两种不同的特性：多能性和分化为各种细胞类型的潜力。因此，这些细胞可作为移植或组织工程应用的细胞来源。我们注意到聚集诱导发射的效应，自组装的能力可以增强信号的持久性。用低浓度的TPE-11纳米颗粒处理后，he细胞显示出高强度的标记，免疫染色分析和畸胎瘤形成试验表明，在这种浓度下，它们的多能性保持不变。此外，这些纳米颗粒可以长期监测hES细胞向神经元样细胞的分化，持续40 天。
Fluorogenic labeling is a potential technique in biology that allows for direct detection and tracking of cells undergoing various biological processes. Compared to traditional genetic modification approaches, labeling cells with nanoparticles has advantages, especially for the additional safety they provide by avoiding genomic integration. However, it remains a challenge to determine whether nanoparticles interfere with cell traits and provide long-lasting signals in living cells. We employed an amphiphilic fluorophore-derived nanoparticle (denoted by TPE-11) bearing a tetraphenylethene (TPE) moiety and two ionic heads; this nanoparticle has an aggregation-induced emission (AIE) effect and the ability to self-assemble. TPE-11 exhibited the property of higher or longer fluorescence intensities in cell imaging than the other two nanomaterials under the same conditions. We used this nanomaterial to label human embryonic stem (hES) cells and monitor their differentiation. Treatment with low concentrations of TPE-11 (8.0 μg/mL) resulted in high-intensity labeling of hES cells, and immunostaining analysis and teratoma formation assays showed that at this concentration, their pluripotency remained unaltered. TPE-11 nanoparticles allowed for long-term monitoring of hES cell differentiation into neuron-like cells; remarkably, strong nanoparticle signals were detected throughout the nearly 40-day differentiation process. Thus, these results demonstrate that the TPE-11 nanoparticle has excellent biocompatibility for hES cells and is a potential fluorogen for labeling and tracking the differentiation of human pluripotent stem cells.Statement of SignificanceThis study uses a nanoparticle-based approach to label human embryonic stem (hES) cells and monitor their differentiation. hES cells are distinguished by two distinctive properties: the state of their pluripotency and the potential to differentiate into various cell types. Thus, these cells will be useful as a source of cells for transplantation or tissue engineering applications. We noticed the effect of aggregation-induced emission, and the ability to self-assemble could enhance the persistence of signals. Treatment with low concentrations of TPE-11 nanoparticles showed high-intensity labeling of hES cells, and immunostaining analysis and teratoma formation assays showed that at this concentration, their pluripotency remained unaltered. Additionally, these nanoparticles allowed for long-term monitoring of hES cell differentiation into neuron-like cells lasting for 40 days.