Towards a microMRI atlas of mouse development
Towards a microMRI atlas of mouse development
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DOI：
10.1016/s0895-6111(98)00059-7
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发表时间：
1999-01-01
影响因子：
5.7
通讯作者：
Laidlaw, D
中科院分区：
文献类型：
--
作者：
Jacobs, RE;Ahrens, ET;Laidlaw, D
本研究探讨了显微磁共振成像的潜力，以获得信息的三维数字地图集的小鼠发展使用固定的样品。固定的样品允许直接与已经发表的图谱进行比较，并为未来的体内研究提供了测试基础。小鼠胚胎的3D MR图像（dpc 6.5-16）表明，该技术可获得必要的对比度和细节水平。扩散加权成像，扩散张量成像，和多值数据集的唯一MR方法获得解剖信息的例子。对完整样本进行非侵入性MRI，在进行MRI实验后，保留其他操作的可能性（例如经典组织学、免疫组织化学、原位杂交和未固定样本的体外生长）（C）1999 Elsevier Science Ltd.保留所有权利。
This study investigates the potential of microscopic Magnetic Resonance Imaging to obtain information for 3D digital atlases of mouse development using fixed samples. Fixed samples allow direct comparison with already published atlases and provide a testing ground for future in vivo efforts. 3D MR images of mouse embryos (dpc 6.5-16) illustrate that the necessary contrast and level of detail is available with this technique. Diffusion weighted imaging, diffusion tensor imaging, and multi-valued data sets are presented as examples of uniquely MR methods of obtaining anatomical information. MRI is performed non-invasively on the intact sample, leaving open the possibility of other manipulations (e.g. classical histology, immunohistochemistry, in situ hybridization, and in vitro growth for unfixed samples) after conducting the MRI experiment (C) 1999 Elsevier Science Ltd. All rights reserved.